体外冲击波对兔膝骨关节炎软骨白细胞介素-1β及肿瘤坏死因子-α表达的影响

Effect of Extracorporeal Shock Wave on Expression of Interleukin-1β and Tumour Necrosis Factor-α in Cartilage in Rabbits with Knee Osteoarthritis

目的观察体外冲击波(ESW)对兔膝骨性关节炎软骨白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平的影响。方法将30只新西兰大白兔随机分为对照组、模型组和治疗组,每组各10只。模型组和治疗组采用改良伸直位固定6周,制备兔膝骨关节炎模型。治疗组造模后立即给予ESW治疗1次,能流密度0.1 mJ/mm2,冲击次数1000次。各组分别于治疗后4周处死,观察大体标本形态学变化,采用免疫组织化学法检测各组关节软骨中IL-1β和TNF-α的水平。结果治疗组IL-1β和TNF-α的表达较模型组明显下调(P<0.01)。结论 ESW能下调兔膝骨关节炎软骨细胞IL-1β、TNF-α的表达。

Objective To explore the effect of extracorporeal shock wave （ESW） on expression of interleukin-1β（IL-1β） and tumor ne-crosis factor-α（TNF-α） in cartilage in rabbits with knee osteoarthritis. Methods 30 rabbits were randomly divided into control group, model group and treatment group, with 10 rabbits in each group. Model of knee osteoarthritis was established with modified plaster cast in exten-sion position for 6 weeks except the control group, and the treatment group was treated with ESW （each 1000 impulse, the energy flux densi-ty of 0.1 mJ/mm2）. The rabbits were sacrificed respectively 4 weeks after ESW treatment, the general and histological changes of articular cartilage were examined and immunohistochemical staining was used to observe the expression of IL-1βand TNF-αin cartilage. Results The expression of IL-1βand TNF-αwere significantly lower in the treatment group than in the model group （P〈0.01）. Conclusion ESW can down-regulate the expression of IL-1βand TNF-αin chondrocytes with osteoarthritis.