缺血性脑卒中大鼠大脑皮层EphrinB2信号表达变化的研究被引量：1

Expression of EphrinB2 Signal in Cerebral Cortex in Ischemic Stroke Rats

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摘要目的观察大鼠缺血性脑卒中后皮层中磷酸化EphrinB2的表达。方法将成年雄性Sprague-Dawley大鼠随机分为假手术组和模型组,每组12只。采用线栓法制作大鼠大脑中动脉阻塞(MCAO)缺血再灌注模型。利用Western blotting和免疫组化SP法检测两组大鼠脑皮层中磷酸化EphrinB2的表达。结果模型组大鼠大脑皮层的磷酸化EphrinB2表达量和阳性细胞数目均高于假手术组(P<0.05),并且血管内皮有磷酸化EphrinB2的表达。结论大鼠缺血性脑卒中后EphrinB2信号通路被激活。 Objective To observe the expression of phosphorylated EphrinB2 in brain after focal cerebral ischemia/reperfusion in rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham group （n=12） and model group （n=12）. The model group was mod-eled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture, and then was assessed with Longa＇s score. The ex-pression of phosphorylated EphrinB2 in cerebral cortex was detected with immunohistochemistry and Western blotting. Results The expres-sion of phosphorylated EphrinB2 and the number of positive cells were significantly higher in the sham group than in the control group （P〈0.05）. It existed in the vascular endothelium in cerebral cortex. Conclusion EphrinB2 signaling pathway is activated in ischemic stroke.

作者郭心磊孙芳玲艾厚喜张丽王文安宜

机构地区北京理工大学首都医科大学宣武医院药物研究室

出处《中国康复理论与实践》 CSCD 北大核心 2014年第2期129-132,共4页 Chinese Journal of Rehabilitation Theory and Practice

基金国家自然科学基金(No.81173575 No.81373994) 北京市自然科学基金(No.7062031) 北京市教委科研基地-科技创新平台(No.111219) 2011年北京市卫生系统高层次卫生技术人才(No.2011-3-097)

关键词脑卒中大脑中动脉阻塞缺血再灌注 EPHRINB2 大鼠 EPHRINB2 stroke middle cerebral artery occlusion ischemia/reperfusion rats

分类号 R743.3 [医药卫生—神经病学与精神病学]

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1Lo EH, Dalkara T, Moskowitz MA. Mechanisms, challengesand opportunities in stroke [J]. Nat Rev Neurosci, 2003, 4(5):399-415.
2Wilkinson DG. Multiple roles of EPH receptors and ephrins inneural development [J]. Nat Rev Neurosci, 2001,2: 155-164.
3Abengozar MA, de Frutos S, Ferreiro S,et al. Blocking eph-rinB2 with highly specific antibodies inhibits angiogenesis,lymphangiogenesis, and tumor growth [J]. Blood, 2012,119(19): 4565-4576.
4Taylor AC, Mendel TA, Mason KE, et al. Attenuation of eph-rinB2 reverse signaling decreases vascularized area and prereti-nal vascular tuft formation in the murine model of oxygen-in-duced retinopathy [J]. Invest Ophthalmol Vis Sci, 2012, 53(9):5462-5470.
5Wang HU, Chen ZF, Anderson DJ, Molecular distinction andangiogenic interaction between embryonic arteries and veins re-vealed by ephrin-B2 and its receptor Eph-B4 [J]. Cell, 1998, 93(5): 741-753.
6Gariano RF, Gardner TW. Retinal angiogenesis in developmentand disease [J]. Nature, 2005, 438(7070): 960-966.
7Palmer A, Klein R. Multiple roles of ephrins in morphogenesis,neuronal networking, and brain function [J]. Genes Dev, 2003,17(12): 1429-1450.
8Mansson- Broberg A, Siddiqui AJ, Genander M, et al. Modula-tion of ephrinB2 leads to increased angiogenesis in ischemicmyocardium and endothelial cell proliferation [J]. BiochemBiophys Res Commun, 2008, 373(3): 355-359.
9Bundesen LQ, Scheel TA, Bregman BS, et al. Ephrin-B2 andEphB2 regulation of astrocyte- meningeal fibroblast interac-tions in response to spinal cord lesions in adult rats [J]. J Neu-rosci, 2003, 23(21): 7789-7800.
10Longa EZ, Weistein PR, Calson S,et al. Reversible middle ce-rebral artery occlusion without craniectomy in rats [J]. Stroke,1989, 20(1): 84-91.