高效液相色谱法分析宫颈癌及宫颈上皮内瘤变患者的血浆氨基酸变化

Analysis of the Blood Amino Acid Metabonomics of Cervical Intraepithelial Neoplasia and Cervical Cancer by High-performance Liquid Chromatography

利用高效液相色谱(HPLC)代谢组学技术检测宫颈癌、宫颈上皮内瘤变及健康人血浆游离氯基酸含量,探讨宫颈癌及宫颈上皮内瘤变患者血浆游离氯基酸含量的改变及其临床意义。收集新疆医科大学第一附属医院病理确诊的26例宫颈上皮内瘤变(CIN)患者、22例宫颈癌(CSSC)患者及35例健康人血液标本,应用偏最小二乘判别分析法(PLS-DA)对HPLC谱数据进行模式识别分析,将其结果与健康人血浆游离氯基酸进行比较。结果表明,与健康人相比,CIN和宫颈癌患者血浆存在明显的氨基酸代谢异常。天冬氨酸、谷氨酸、天冬酰胺、丝氨酸、甘氨酸、组氨酸、牛磺酸、丙氨酸、脯氨酸、酪氨酸、缬氨酸、蛋氨酸、异亮氨酸、亮氨酸、赖氨酸、苯丙氨酸含量自健康人、宫颈上皮内瘤变到宫颈癌呈下降趋势,且以宫颈癌血浆氨基酸含量下降最为显著,差异具有统计学意义(P<0.05);而精氨酸和苏氨酸在宫颈上皮内瘤变血浆中较健康人明显增高,差异具有统计学意义(P<0.05)。由此得出,肿瘤细胞可能选择性地从血浆中摄取特定氨基酸来满足自身不同阶段的生长需求。

To investigate the amino acids in plasma samples from patients with the cervical intraepithelial neoplasia （CIN） and the cervical cancer and from healthy volunteers, to assess their clinical significance and to have insights into the cervical cancer amino acid metabolism and the systemic effects, 26 plasma samples from the CIN patients, 22 plasma samples from the CSCC patients and 35 samples from healthy volunteers are analyzed by the HPLC, and the partial least-squares discriminant analysis （PLS-DA） is employed to perform the pattern recognition analysis of the HPLC spectra. It is shown that there is a significant difference in plasma amino acids between those from patients with the CIN and cervical cancers and from healthy subjects. Compared to the healthy controls, the plasma aspartate, the glutamate, the asparagine, the serine, the glycine, the histidine, the taurine, the alanine, the proline, the tyrosine, the valine, the methionine, the lysine, the isoleucine, the leucine, and the phenylalanine decrease gradually from the CINto cervical cancers with statistical significance （P〈0.05）, however the arginine and the threonine decrease dominantly in the CIN plasma than those in the CSCC. These findings indicate that the tumor cells may uptake a specific amino acid selectively from the plasma to meet their needs at different stages of growth.