刚毛藻对重金属Pb^(2+)的耐受性及吸附性研究

Study on Characteristics of Tolerance and Adsorption of Chadophorasle to Pb^(2+)

[目的]研究重金属Pb2+对刚毛藻的损害情况以及刚毛藻对重金属Pb2+的吸附性能。[方法]将刚毛藻置于不同浓度(0、2.5、5.0、7.5、10.0、12.5、15.0、17.5、20.0 mg/L)重金属Pb2+的培养液中进行培养,培养周期为10 d,测定分析刚毛藻体内的叶绿素含量、丙二醛(MDA)含量、蛋白质含量及刚毛藻体内重金属Pb2+的含量。[结果]随着重金属Pb2+浓度的增大,刚毛藻体内叶绿素含量急剧下降,最终维持在0.30 mg/mg左右,Ca/Cb比值逐渐减小最终维持在0.30左右,重金属Pb2+对叶绿素a的损害作用随着浓度的增大而逐渐增大,且对叶绿素a的损害作用要大于对叶绿素b的损害作用。重金属Pb2+浓度在0~20 mg/L时,刚毛藻体内的MDA含量呈持续上升趋势,最大值为20.44μmol/g,表明重金属Pb2+对刚毛藻的毒害作用在逐渐增大。随着重金属Pb2+浓度的增加,刚毛藻体内的蛋白质含量整体呈下降趋势,最终维持在1.10 mg/g。推测刚毛藻对重金属Pb2+的吸附分为两个阶段,即快速吸附阶段和缓慢吸附阶段。[结论]刚毛藻对重金属Pb2+具有一定的吸附能力及耐受性,可以用于控制并修复水体中的重金属。

[ Objective ] The aim was to study the damage situation of Pb2＋ to ehadophorasle, and the characteristics of adsorption of chadophorasle to Pb2＋. [ Method] Different concentrations（0, 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 17.5, 20.0 rag/L） of Pb2 ＋ was used to train chadophorasle, time of the test was 10 d, the contents of chlorophyll, MAD and protein were determined and analyzed. [ Result] With increasing of Pb2＋ concentration, the chlorophyll contents of ehadophorasle decreased sharply, finally retained at about 0.30 rag/rag, the ra- tion of Ca to Cb decreased gradually, and finally retained at about 0.30, so the damage of Pb2＋ to chlorophyll a increased with its concentra- tion rose, and the damage to chlorophyll a was bigger than that of to chlorophyll b. When Pb2＋ concentration was 0-20 rag/L, the MDA con- tent of chadophorasle had continuous rising tendency, and the maximum was 20.44 μmol/g, showed that the toxic action of Pb2 ＋ to chadopho- rasle increased gradually. With increasing of Pb2＋ concentration, the protein content of chadophorasle had decreasing tendency in a whole, and retained 1.10 mg/g. It was supposed that the adsorption action of ehadophorasle to Ph2＋ had two periods, namely fast adsorption period and slow adsorption period. [ Conclusion] Chadophorasle has some adsorptive ability and tolerance to Pb2＋ , and can be used to control and re- pair the heavy metal in water.