摘要
目的探讨应用玫瑰花结法与流式细胞术检测T细胞亚群,比较两者科学性与实用性。方法分别通过玫瑰花结法及流式细胞仪检测160例健康人外周血中T细胞亚群变化情况,比较CD3+、CD4+、CD4+/CD8+、CD8+水平、检测费用、检测时间。结果两种方法检测后CD3+、CD4+、CD4+/CD8+、CD8+水平比较无显著性差异(P>0.05),玫瑰花结法的检测时间、费用均明显少于流式细胞术,差异具有显著性(P<0.01)。结论与流式细胞术相比,玫瑰花结法检测T细胞亚群操作简单、快捷、准确率高,值得临床广泛应用。
Objective To detect T cell subsets of peripheral blood by rosette method and flow cytometry and compare the two methods. Methods Rosette method and flow cytometry assay were used respectively for the detection of peripheral blood T cell subsets of CD3 ＋ , CD4 ＋ , CD4 ＋/CD8 ＋ and CD8 ＋ in 160 healthy subjects. The results of the detection, and cost and time of detection were compared between the two methods. Results There were no differences in the levels of CD3＋ , CIM＋ , CIM＋/CD8＋ and CD8＋ between two methods （ P 〉0.05） ; how- ever, the differences in the time and cost of two detection method was significant （ all P 〈 0.01 ）. Conclusion Compared with flow cytometry, rosette method is simple, fast and accurate for the detection of T cell subsets.
出处
《临床和实验医学杂志》
2014年第3期224-226,共3页
Journal of Clinical and Experimental Medicine
