β_2肾上腺素能受体基因多态性与汉族人群早产的相关性研究

Association between Genetic Polymorphisms of β_2-adrenergic Receptor Gene and Preterm Delivery in Han Population

目的:研究β2肾上腺素能受体(β2-AR)基因多态性与汉族人群早产的相关性。方法:将236例产妇分为两组,研究组129例,早产产妇为79例,妊娠过程中发生过先兆早产的产妇为50例;对照组107例为妊娠期间未曾保胎的足月产妇。收集其外周静脉血,提取DNA,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测β2-AR基因编码第16和27位氨基酸的基因多态性。结果:β2-AR基因编码第16位氨基酸基因型AA、GG、AG频率在研究组和对照组分别为36.43%、18.60%、44.96%和34.58%、15.89%、49.53%;编码第27位氨基酸基因型NN、NU频率在研究组和对照组分别为77.52%、22.48%和78.50%、21.50%,UU基因型未检出;两位点基因型分布频率差异均无统计学意义(P>0.05)。结论:β2-AR基因编码第16和27位氨基酸的基因多态性与早产的发生无相关性,提示该多态位点并不是引起早产的独立危险因素。

OBJECTIVE： To study the correlation between gene polymorphism of β2-adrenergic receptor and preterm delivery in Han population. METHODS： DNA was extracted from the blood samples taken from 236 delivery women, among whom there were 129 cases in study group, including 79 cases gave premature births and 50 cases suffered from threatened premature labor; there were 107 normal full term pregnant women without tocolysis treatment in control group. Then PCR-RFLP was performed to determine the gene polymorphism of β2-adrenergic receptor （Arg16Gly and Gln27Glu）. RESULTS： The frequencies of Arg16Gly genotype AA, GG, AG were 36.43% , 18.60% , 44.96% and 34.58% , 15.89% , 49.53% , respectively. The frequencies of Gln27Glu genotype NN and NU were 77.52%, 22.48% and 78.50%, 21.50%, respectively; UU genotype was not detected. There was no statistical significance （P〉0.05）. CONCLUSIONS： These data show that Argl6Gly and Gln27Glu polymorphism of β2-AR gene may not correlate to preterm delivery, which indicates polymorphic site is not independent risk factor of premature birth.