小半夏加茯苓汤中鸟苷和腺苷含量的高效液相色谱法测定

Determination of guanosine and adenosine in Xiaobanxiajiafuling Decoction by HPLC

目的建立测定小半夏加茯苓汤中乌苷和腺苷含量的HPLC分析方法。方法色谱柱为Diamonsil C18柱（4．6mm×250m／F／，5μm），流动相为乙腈－0．01％醋酸水溶液，梯度洗脱，检测波长25411171，体积流量为1．0ml·min^-1，柱温30℃。结果鸟苷和腺苷分别在0．031—1．027μg（r=0．9999）和0．015—1．010μg（r=1．0000）内线性关系良好，平均加样回收率分别为102．8％、102．5％，RSD分别为1．93％、1．8％（n=6）。结论该方法简便、可靠、重现性好，可用于测定小半夏加茯苓汤中鸟苷和腺苷的含量。

Objective To establish an HPLC method for the content determination of guanosine and adenosine in Xiaobanxiajia- fuling Decoction. Methods The Diamonsil Cls column was used. The mobile phases consisted of acetonitrile and 0.01% ethanoic acid with gradient elution. The detection wavelength was at 254 rim. The flow rate was 1.0 ml. min^ -1 and column temperature was at 30 ℃. Results Guanosine and adenosine were linear in range of 0. 031 - 1. 027 μg （r = 0. 999 9） and 0. 015 - 1. 010 μg （r = 1. 000 0）respectively. The average recoveries were 102.8% and 102.5% with the RSD 1.93% and 1.8% （n = 6） respectively. Conclusion The method is rapid, accurate, reliable and can be used to determine the guanosine and adenosine in Xiaobanxiajiafuling Decoction.