胡芦巴总皂苷的提取纯化工艺考察

Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen

目的:制备高纯度的胡芦巴总皂苷。方法:采用乙醇回流法提取胡芦巴总皂苷,依次加正己烷、三氯甲烷、乙酸乙酯、正丁醇进行系统溶剂萃取,三氯甲烷有效部位经HPD-400型大孔吸附树脂富集纯化。采用显色反应和TLC鉴别总皂苷,运用UV测定胡芦巴总皂苷含量。结果:三氯甲烷萃取部位中总皂苷纯度40%,得率6.28%;水洗脱液中胡芦巴总皂苷纯度80.5%,得率0.32%。结论:优选的提取纯化工艺快速简便,为胡芦巴总皂苷的药效学评价奠定基础。

Objective：To prepare total saponins of Trigonellae Semen with high purity.Method：Total saponins of Trigonellae Semen was extracted by ethanol refluxing method,then extract was dealt with different solvents systematically,including n-hexane,chloroform,ethyl acetate,n-buthanol.Chloroform effective part was purified through HPD-400 macroreticular resin.Total saponins in each steps and fractions was identified by chromogenic reaction and TLC,contents of total saponins were determined by ultraviolet spectroscopy.Result：Purity of total saponins in chloroform solvent extraction was 40％ with yield of 6.28％ ; Purity of total saponins in water eluent was 80.5％ with yield of 0.32％.Conclusion：This optimized process was convenient and efficient,it could lay foundation for pharmacodynamic study.