HPLC同时测定黄连阿胶汤合煎液中芍药苷黄芩苷小檗碱的含量

Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC

目的:建立高效液相色谱法同时测定黄连阿胶汤中芍药苷、黄芩苷、小檗碱3种成分的含量。方法:采用Agilent TC-C18色谱柱(4.6 mm×250 mm),流动相乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 mL·min-1,检测波长240 nm,柱温25℃。结果:黄连阿胶汤中芍药苷、黄芩苷、小檗碱平均质量分数分别为1.64%,7.51%,3.30%。结论:该法操作简便、准确且精密度和重复性良好,可用于黄连阿胶汤的质量控制。

Objective：A simple and rapid HPLC method was established for simultaneously determining of paeoniflorin,baicalin and berberine in the mixed decoction of Huanglian Ejiaotang.Method：Agilent TC-C18 （4.6 mm× 250 mm） chromatographic column,acetonitrile-0.1％ phosphoric acid solution gradient elution were applied.Analyses were performed at 35 ℃ with a flowrate of 1.0 mL·min-1 and UV detection at 240 nm,Result：The content of paeoniflorin,baicalin and berberine were 1.64％,7.51％,3.30％ respectively.Conclusion：The developed method can be applied to the intrinsic quality control of Huanglian Ejiaotang.