摘要
目的:分析五味子苦味物质组成及其生物活性。方法:采用有机溶剂提取,柱色谱分离的方法制备五味子苦味物质,以HPLC对其进行定性定量分析并观察苦味物质的生物活性。结果:苦味物质主要含有木脂素类成分,五味子醇甲等6种木脂素总量约为42.5%。苦味物质高剂量组能对小鼠自主活动有明显抑制作用(P<0.05);高、低剂量组均能显著延长戊巴比妥钠小鼠的睡眠时间(P<0.001);苦味物质对小鼠负重游泳时间无明显影响,但可以延长其耐常压缺氧时间(P<0.05);苦味物质高、低剂量组均能降低高血脂大鼠的总胆固醇(TC)含量(P<0.01),升高低密度脂蛋白(LDL)和高密度脂蛋白(HDL)的含量,且高剂量组作用强于低剂量组。结论:五味子苦味物质是其镇静、催眠、抗缺氧及降血脂作用的物质基础。
Objective:Reveal the composition of bitter substance and its biological activity of Schisandra chinensis.Method:Using organic solvent extraction and silica gel separation to prepare bitter substances from Schisandra chinensis,HPLC were applied for qualitative and quantitative analysis.Meanwhile,observed the biological activity of bitter substance.Result:The bitterness substances mainly contains lignans,the total amount of six lignans were approximately 42.5%.The bitterness substances in high dose group had significant inhibitory effect on the spontaneous activity of mice (P < 0.05) and also could significantly prolong the sleeping time of mice with pentobarbital sodium (P <0.001) ; and there has no obvious effect on the swimming time of mice,but can prolong the normobaric hypoxia tolerance time (P < 0.05) ; high and low groups bitter substance can reduce total cholesterol in hyperlipidemic rats (TC) (P < 0.01),elevated low density lipoprotein (LDL) and high density lipoprotein (HDL) content,and high dose group was stronger than the low dose group.Conclusion:Bitter substance of S.chinensis is the Pharmacodynamic substance with sedative,hypnotic,antihypoxia and hypolipidemic effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第5期110-113,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然基金面上项目(30973874)
中医药行业专项(201007012-1)
关键词
五味子
苦味物质
镇静
催眠
抗缺氧
降血脂
Schisandrae chinensis
bitter substance
sedation
hypnosis
anti-hypoxia
reducing blood lipid