基于DNA水平的中国药用植物分子鉴定方法

DNA-based Authentication of TCM by Molecular Biological Techniques

通过对近年来国内外基于DNA水平分子鉴定方法研究的文献回顾,作者整理了几种经典的基于DNA水平的中药分子鉴定方法,包括RFLP,DNA microarray,RAPD,CAPS,ISSR,SSR等。随着分子生物学的迅速发展,DNA分子标记技术日趋成熟,该领域出现了诸多分子标记技术,并且已经能够很好的应用到中药基源鉴定中。最近兴起的DNA barcoding和DNA chip技术在DNA分子标记领域亦显示出了极大的优越性和潜力,结合即将出现的新的分子生物学分析方法笔者做了相应的阐述。任何一种药材的鉴定方法都存在局限性,重要的是要充分了解和应用其优势,从而合理和有效地对现有方法进行补充和改进,随着生物分子领域的迅速发展,有理由相信DNA分子鉴定方法值得进一步的研究和开发。

Recently,molecular biotechnology has provided sophisticated molecular techniques for authentication of botanical materials at the DNA level and DNA technology provides a powerful tool to complement chemical analyses for authentication of Chinese medicinal plants and to ensure that herbal materials are not contaminated with ineffective or potentially harmful substitutes or adulterants.this review provides an account of commonly used DNA-based technologies [ex：RFLP,AFLP,ISSR,SSR,CPS,RAPD] for TCM plants.Recent achievements in the field of DNA barcoding and DNA chip technology that offfer great potentials for detecting of DNA and emerging new developments for future identification of species are briefly outlined.