罗非鱼内脏蛋白酶解超滤产物的抗氧化活性研究

Antioxidant activity of tilapia viscera hydrolysates and ultrafiltration products

将罗非鱼内脏蛋白水解得到可溶性多肽,利用超滤技术对水解产物进行分级分离,并检测酶解产物及其超滤产物的体外还原能力、清除DPPH自由基能力、清除超氧阴离子能力及抑制卵黄磷脂氧化能力。结果表明:酶解可以得到分子量范围宽、具有多种抗氧化能力的多肽;超滤分级后,分子量<5kDa的多肽组分抗氧化能力最强,尤其是该组分清除超氧阴离子的能力及抑制脂质过氧化的能力接近VC,其IC50值与VC的IC50值处于同一个数量级。

Tilapia viscera was hydrolyzed, ultra filtrated; and then soluble peptides were obtained. The antioxidant activities in vitro of the hydrolysates were evaluated in four respects by determination of the reducing power, DPPH radical scavenging activities, scavenging hydroxyl radical activities, and inhibition of lipid peroxidation in yolk lecithin. Results indicated that broad molecular weights of polypeptides with multiple antioxidant capacities were produced after enzymolysis. Upon ultra filtration, peptides molecular weight 〈 5 kDa demonstrated the strongest antioxidant capacity, especially for its scavenging hydroxyl radical activity and lipid peroxidation. Both of IC50values were at the same level of that of VC.