简青霉对水溶液中碱木质素的吸附和降解的机理

Mechanism of adsorption and degradation of alkali lignin in aqueous solution by Penicillium simplicissimum

通过一系列实验研究碳源浓度、培养箱转速、碱木质素浓度以及菌体活性等因素对简青霉菌丝球在溶液中吸附碱木质素的影响,并探讨其生物吸附机理;确定最佳碳源浓度和转速,分别为10 g/L和150 r/min。当碱木质素浓度小于0.5 g/L时,吸附效果较好,由菌丝球活性对照实验以及电镜照片可知吸附作用主要是由菌丝球的物理结构决定的,与其是否具有活性关系不大。通过吸附等温实验确定菌丝球的最大吸附量为30.3 mg/g,且吸附过程符合Langmuir等温吸附模型。另外,过氧化氢对木质素降解酶影响的实验证明,木质素过氧化物酶和锰过氧化物酶均需要过氧化氢的启动才能催化降解木质素,并且在降解过程中,木质素过氧化物酶和锰过氧化物酶的作用远远大于漆酶。

A test was conducted for the adsorption of the alkali lignin by Penicillium simplicissimum through a series of condition ang analysis experiments. The results indicated that the optimized condition could be obtained when carbon source concentration was kept at 10 g/L,as the rotating speed of incubator was maintained at 150 r/min and the alkali lignin concentration was less than 500 mg/g. The adsorption efficiency had nothing to do with the activity of the fungi. The whole adsorption process conformed to the Langmuir model,and the maximum adsorption was 30. 3 mg/g. In addition,the effects of adding H2O2 on ligninolytic enzymes were analyzed,the results indicated that lignin peroxidase and manganese peroxidase catalyzed the degradation of lignin only when H2O2 was presenced,and they were much more important than laccase during the degradation process of alkali lignin.