微卫星位点AciG-35在中华鲟野生个体中不同等位基因的序列变异研究

Allele Variation of a Microsatellite Locus AciG-35 in Individuals of Wild Chinese Sturgeon

本研究利用PCR扩增和序列测定得到了1个特殊4碱基重复微卫星位点AciG-35在中华鲟野生个体中的34个等位基因序列。根据序列测定的结果,初步研究了该位点的核心重复区序列以及两端侧翼序列的变异情况。该位点不同等位基因的核心序列和侧翼序列都表现出一定程度的变异,其中核心重复序列主要表现为点突变,侧翼序列主要表现为各种碱基的替换、插入和缺失以及片段的插入缺失。此外,还发现了AciG-35引物在中华鲟中的多位点扩增现象。研究结果对于准确解释微卫星分子标记用于群体遗传分析时的数据结果具有重要意义,对在微卫星标记的应用过程中可能存在的一些问题一并进行了探讨。

In this study, 34 allele sequences at a special tetranucleotide repeat microsatellite locus AciG-35 were obtained in the individuals of wild Chinese sturgeon. From the sequencing results, a preliminary study was performed on the variation of the repeat region of the core sequences and the flanking sequences at both ends. There was a certain degree of variability in the core and and the flanking sequences of different alleles, which reflected as point mutations in the core region, and a variety of nucleotide substitutions, insertions and deletions as well as fragment indels in the flanking sequences. In addition, we also found multi-locus amplification of the primer for AciG-35 locus in Chinese sturgeon. The results would be important for the accurate interpretation of microsatellite DNA markers for population genetic analysis data. At the same time, some of the problems that may exist in the application process of microsatellite markers were discussed here.