人参皂甙肠道代谢物CompoundK对人胃癌细胞增殖及凋亡相关基因表达的影响

Compound K induced apoptosis of gastric carcinoma cells and the effect on expression of apopto-sis-related genes

目的观察人参皂甙肠道代谢物CompoundK（CK）对人胃癌细胞BGC823细胞增殖、凋亡的影响。方法实验分组：对照组（PBS溶液），实验组（浓度分别为2．5、5、7．5、10μmol／L）。噻唑蓝（MTT）比色法分别检测CK作用于细胞后其活力及生长抑制率；流式细胞术检测CK作用后细胞周期时相、凋亡率；免疫印迹法（Westernblot）检测凋亡相关蛋白的表达。结果5μmol／LCK作用细胞48h时，细胞生长周期阻滞于G2／M期，细胞凋亡率为（21．17±3．16）％；Westernblot检测显示Bcl-2，Bid蛋白表达随着药物浓度的增加而减少，Fas和Fas—L蛋白表达量却没有明显变化。结论CK是通过线粒体介导的凋亡通路途径来诱导人胃癌细胞发生凋亡的。

Objective To investigate the effect of CK on proliferation, apoptosis of BGC823 cells. Methods BGC823 cells were treated with CK at different concentrations, cell viability was as-sayed using MTT methods. Cell cycle, apoptosis rate were assayed by flow cytometry. The expression of apoptosis-related protein were detected by Western blotting. Results After BGC823 cells were treated with 5 μmol/L CK for 48 h, the growth cycle was arrested on G2/M phase, the apoptosis rate was （21.17 ± 3.16）%. The expressions of Bcl-2, Bid protein was down-regulal the Fas and Fas-L protein were not significantly changed. Conclusions BGC823 cells, and induces apoptosis via mitochondrion-mediated pathway Lted after CK treatment, while CK inhibits proliferation of BGC823 cells, and induces apoptosis via mitochondrion-mediated pathway.