高粱原花青素四聚体干预Streptococcus mutans Ingbritt(c)体外黏附途径及机理

in vitro Adhesion Pathways and Mechanisms of Streptococcus mutans Ingbritt(c) during Intervention with Proanthocyanidins Tetramers

以高纯度高粱原花青素(sorghum procyanidins,SPC)混合物和高粱原花青素四聚体(sorghum procyanidins tetramers,SPC-Ⅲ)为原料,采用荧光标记的方法比较其抑制Streptococcus mutans Ingbritt(c)黏附效果,在此基础上结合双向凝胶电泳(two-dimensional electrophoresis,2-DE)以及基质辅助激光解吸电离飞行时间质谱(matrix-associated laser dissociation/ionization time of fl ight mass spectrometry,MALDI-TOF-MS)探究其干预S.mutans Ingbritt(c)黏附的主要途径并期望阐明其作用机理。结果表明:SPC-Ⅲ比SPC混合物抑制S.mutans Ingbritt(c)体外黏附的效果更强,SPC-Ⅲ处理细菌的抑制效果强于处理获得性膜的抑制效果,是其抑制细菌黏附主要途径;类葡萄糖基转移酶(glycosyltransferases,GTFs)活性蛋白(3.10-Ⅱ)经SPC-Ⅲ作用前后的2-DE图有明显差异,差异点均为S.mutans中的不同蛋白酶类,其中A24可能是一种新蛋白。

In this study, high purity proanthocyanidins（SPC） and proanthocyanidins tetramers（SPC-III） from sorghum episperm were compared for their inhibitory effects on the adhesion of Streptococcus mutans Ingbritt（c） through fluorescence labeling. By combining two-dimensional electrophoresis（2-DE） and matrix-associated laser dissociation/ ionization time of flight mass spectrometry（MALDI-TOF-MS）, the major intervening pathways were investigated, and the underlying mechanisms were explored. The results showed that SPC-III（tetramers） had better inhibitory effect than SPC and had stronger inhibitory effect than SPC-III-treated salivary acquired pellicle on the adhesion of S. mutans Ingbritt（c）, which was the major pathway to inhibit bacterial adhesion. Two-dimensional electrophoretograms of 3.10-II treated by SPC-III or not showed significant differences. All different points were different proteases derived from S. mutans, in which A24 was suggested to be a new protein that has never been detected in S. mutans previously.