响应面法优化金银花多酚氧化酶提取工艺

Optimization of Extraction Process for Polyphenoloxidase from Honeysuckle(Lonicera japonica Thunb.) Flowers Using Response Surface Methodology

以金银花为原料,采用匀浆浸提法提取多酚氧化酶,利用单因素试验和响应面优化法对料液比、提取pH值、聚乙烯吡咯烷酮(polyvinylpyrrolidone,PVP)添加量和浸提时间等工艺条件进行分析与优化。结果表明:匀浆浸提工艺参数对金银花多酚氧化酶提取有显著影响,影响显著顺序为缓冲液pH值>料液比>浸提时间>PVP添加量。金银花多酚氧化酶匀浆浸提优化工艺参数:料液比1∶6.29(m/V)、缓冲液pH 8.03、PVP添加量1 g/100 mL、浸提时间1.71 h,在此条件下获得酶比活力为667.564 U/mg,所得多酚氧化酶提取回归模型显著(R2=0.9425),拟合性好,可用于预测多酚氧化酶的提取效果。

This study was aimed to optimize the extraction process for polyphenoloxidase（PPO） from honeysuckle（Lonicera japonica Thunb.） flowers using response surface methodology. The results showed that four operating parameters including buffer pH, material-to-liquid ratio, extraction time and polyvinylpyrrolidone（PVP） concentration had notable effects on the extraction of PPO from honeysuckle flowers and their effects could be ranked in decreasing order of importance as follows： buffer pH ＆gt; material-to-liquid ratio ＆gt; extraction time ＆gt; PVP concentration. The optimal extraction parameters were determined as pH 8.03 and 1：6.29（m/V） of material-to-liquid, 1 g/100 mL PVP added to the extraction solvent and extraction for 1.71 h, resulting in a PPO specific activity of 667.564 U/mg. The established regression model describing PPO extraction from honeysuckle flowers as a function of the four extraction parameters was highly significant（R2=0.9425）. The predicted and experimental results were found to be in good agreement. Thus, the model can be applicable for the prediction of PPO extraction from honeysuckle flowers.