五爪龙外敷液中芹菜素与槲皮素的HPLC测定

Simultaneous Determination of Apigenin and Quercetin in External Application Liquid of Potentilla kleiniana Wight et Arn by HPLC

本实验建立芹菜素与槲皮索的HPLC检测法，同时测定草药五爪龙外敷药液中芹菜素与槲皮素的含量。采用反相shim．packVP．ODSC18色谱柱（4．6mm×150mm，5μm），优化条件为：柱温30℃，流动相：甲醇-0．4％磷酸（50：50，v：v）；流速：1．0mL／min；检测波长：360nm；进样体积10此。对草药五爪龙外敷药液中芹菜素与槲皮素的含量进行测定。结果表明：芹菜素与槲皮素均在60～300μg／mL的范围内线性关系良好，r分别为0．9998与0．9996，草药五爪龙乙醇浸液较水煎煮液中芹菜索与槲皮素含量均高。本法方便、快速、准确，可检测草药五爪龙外敷液中的芹菜素与槲皮素的含量。

The objective of this study was to establish a HPLC method for the simultaneous determination of apigenin and quereetin in the external application liquid of Potentilla kleiniana Wight et Am. Reversed phase ehromatographie column Shim-pack VP-ODS C18（4.6 × 150 mm,5 μm） was used for the separation of apigenin and quereetin under the condi- tions of methanol-4% phosphate acid（50：50 ,v： v） as the mobile phases ,column temperature of 30 ℃ ,flow rate of 1.0 mL/min,detection wavelength at 360 mn and injection volume of 10 μL. The results indieated that both apigenin and quercetin had good linear relationship in the range of 60-300 μg/mL with correlation coefficients of 0. 9996 and 0.9997 respectively. The contents of apigenin and quereetin of the aleohol extraet of P. kleiniana were higher than that of water- deeoeted liquid extract. In eonelusion,the HPLC method developed in this study was convenient, quick and aeeurate. It can be used for determination of apigenin and quereetin in P. kleiniana.