N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响

Effects of N-carbamyglutamate on Intestinal Microflora of Weaned Huanjiang Mini-pigs

研究N-氨甲酰谷氨酸(NCG)对断奶仔猪肠道微生物区系的影响。试验选用体重为3.17±0.21 kg的21日龄断奶环江香猪12头,随机分为2组,每组6头,分别饲喂添加0.1%NCG日粮和基础日粮,试验期为14d。试验结束时,采集回肠和盲肠内容物,利用T-RFLP、PCR/DGGE和荧光定量PCR技术,测定其肠道微生物区系的变化。结果表明,与对照组相比,NCG组回肠内容物中微生物的多样性增加,回肠和盲肠内容物中双歧杆菌和乳酸杆菌的数量显著增加、大肠埃希菌的数量显著降低(P<0.05),盲肠内容物中链球菌的数量也显著降低(P<0.05)。可见,日粮中添加0.1%NCG有助于提高断奶环江香猪肠道内容物中有益菌的数量、抑制有害菌的繁殖,从而改善肠道微生物区系。

This study was conducted to investigate the effects of N-carbamoylglutamate （NCG） on intestinal microflora of weaned mini-pigs. Twelve Huanjiang mini-pigs（3.17 ±0.21 kg of average body weight;weaned at 21 days of age） were assigned randomly into two groups with six replicates in each group. The pigs were fed with a basal diet or 0. 1% NCG- supplemented diet for 14 days. At the end of the trial,the contents of ileum and cecum were collected to determine the intestinal microflora by T-RFLP,PCR/DGGE and RT-qPCR technique. The results showed that diet supplemented with NCG increased（P 〈 0.05） the intestinal microflora diversity in ileum, and population of Bifutobacterium and Lactobacil- lus in ileum and cecum, while decreased（P 〈 0.05 ） Escherichia in ileum and cecum and Streptococcus in cecum when compared with the control group. These findings suggested that diet supplemented with NCG enriched beneficial intesti- nal microflora, but suppressed bacterial pathogens in Huanjiang mini-pigs ,which effectively promoted the diversity of in- testinal mieroflora.