小麦品种抗条锈病基因Yr10、Yr18及1BL/1RS易位的分子检测

Molecular detection of Yr10 and Yr18 genes and 1BL/1RS translocation in wheat cultivars

利用抗条锈病基因Yr10、Yr18以及1BL/1RS易位的SCAR或STS标记,对2006-2010年75份国家审定的小麦品种进行了分子检测,以明确Yr10、Yr18以及1BL/1RS易位在我国2006-2010年审定小麦品种资源中的分布。结果显示:75份材料中有13份检测到Yr10基因的标记,1份检测到Yr18基因的标记,分别占参试材料的17.3%和1.3%,只有‘西农928’能同时检测到Yr10和Yr18基因;25份含有1BL/1RS易位片段,占参试材料的33.3%。表明1BL/1RS易位在我国小麦育种中利用率仍然较高,对目前流行条锈菌小种有良好抗性,而表现慢锈性的Yr18在小麦育种的利用率较低,建议在我国小麦育种中加强利用。

Using SCAR or STS molecular markers, 75 Chinese commercial wheat cultivars were detected for the existence of stripe rust-resistant genes YrlO and Yr18 and 1BL/1RS translocation from 2006 to 2010. The results showed that there were 13 cultivars containing Yr10 gene, accounting for 17.3% of the total tested cultivars, only 1 cultivars with Yr18, accounting for 1.3%, and 25 cultivars with 1BL/1RS translocation, accounting for 33.3%. Wheat cultivar ＇Xinong 928＇ was found to contain both YrlO and Yr18. 1BL/1RS translocation, which is resist- ant to most popular stripe rust races, has been widely used in wheat breeding programs. The slow rust resistance gene Yr18 was seldom used in wheat breeding. Therefore, we suggest that the application of Yr18 gene be in- creased.