异噁草松降解酶在不同条件下降解效果的比较研究

Comparative studies on the degradation effects of the clomazone degradation enzyme under different conditions

为了验证异噁草松高效降解菌W2的降解效果,应用气相色谱法检测异噁草松含量,考察了在室内条件下不同培养温度、土壤湿度、原酶液添加量和异噁草松初始添加浓度对酶液降解异噁草松的影响。结果表明:在培养温度为30℃、土壤湿度为田间最大持水量的50%、原酶液添加量为2mL/kg且酶活力为1.066μg/mL·min的条件下,30d降解酶对异噁草松的降解率最高,达到81.7%,与添加菌悬液处理和药剂对照相比分别提高了31.0%和58.5%,降解半衰期(12.3d)比菌悬液和药剂对照分别缩短了16.9d和68.3d,而异噁草松初始添加浓度对降解酶的降解效果影响差异不显著。

To validate the high efficiency of clomazone degradation by the bacterium W2, this study employed gas chromatography to determine the amount of clomazone. To determine the effects of the clomazone-degrading en- zyme, four factors were studied, involving the temperature, the soil moisture, enzyme addition and clomazone concentration under indoor conditions. The results showed that the best degradation rate of clomazone was 81.7% after 30 d at 30 ℃, 50% of soil water content, 2 mL/kg enzyme and 1. 066μg/mL ·min of enzyme activity, which was 31.0% and 58.5% higher than those of bacterial and reagent treatments, while the degradation half- life was 12.3 d, shortened by 16.9 d and 68.3 d, respectively. There were no significant effects at different clom- azone concentrations on clomazone degradation by the enzyme.