地黄对百草枯解毒机理的研究

Detoxication mechanism of Rehmannia glutinosa to paraquat

地黄对百草枯表现出较强的耐药性,百草枯分别处理300g/hm2或600g/hm2时,药害等级分别为0级和2级,且药害症状恢复较快。研究结果表明,地黄块根、叶片乙醇提取物的DPPH自由基清除率随着提取物浓度的增加而增高;叶片对DPPH自由基的清除效果好于块根,DPPH自由基清除率为50%时所需的地黄块根、叶片乙醇提取物浓度分别为20.0g/L和12.2g/L。可以推测,地黄对百草枯的解毒是因为体内含有抗氧化活性物质,这种物质遇到百草枯时能够解除百草枯活性氧毒性,起到地黄对百草枯的解毒作用。

Rehmannia glutinosa showed tolerance to paraquat. The phytotoxicity of R. glutinosa was grade 0 and grade 2 with paraquat at 300 g/hm2 or 600 g/hm2, respectively. The results showed that the DPPH free radical scavenging activity of ethanol extract from R. glutinosa root tubers and leaves increased with the extractive con- centration adding; the DPPH scavenging effect in the leaves was better than that in the root tubers. The concen- trations of R. glutinosa root tubers and leaves extracted with ethanol were 20.0 g/L and 12.2 g/L, respectively, when the DPPH free radical scavenging activity was 50%. In general, the detoxication of R. glutinosa to paraquat was caused by the antioxidant substance which could remove paraquat reactive oxygen toxicity.