直接竞争酶联免疫吸附分析法测定桃中氰戊菊酯的残留量

Determination of fenvalerate residue in peach by direct competitive enzyme-linked immunosorbent assay

采用包被抗体、酶标半抗原直接竞争酶联免疫吸附分析法（ELISA）测定了氰戊菊酯在桃中的残留量。结果表明：在氰戊菊酯多克隆抗体包被浓度为4．0mg／L、辣根过氧化物酶（HRP）标记氰戊菊酯半抗原稀释1．0×10^5倍条件下，ELISA法检测氰戊菊酯的线性浓度范围为0．01-10mg／L，氰戊菊酯对抗体．酶标半抗原反应的抑制中浓度（IC50）为193μg／L，相对标准偏差（RSD，n=5）为4．5％，IC20为13．5μg／L。在2、0．2和0．05mg／kg添加水平下，ELISA法测定桃中氰戊菊酯的回收率分别为81％-89％、85％～98％和85％-106％，RSD（n=5）分别为5．1％、5．6％和7．7％。ELISA法对桃中氰戊菊酯的最低检出浓度为0．014mg／kg。

The method of antibody immobilized direct competitive enzyme-linked immunosorbent assay （ELISA） was employed to determine fenvalerate residue in peach. The result showed that the linear concentration of ELISA ranged from 0.01 to 10 mg/L, the half maximal inhibitory concentration （IC50） of fenvalerate to antibody hapten-HRP reaction was 193 μg/L with relative standard deviation （RSD, n = 5 ） of 4.5% and the IC20 was 13.5 μg/L. The fenvalerate recoveries from peach determined by ELISA were 81% -89%, 85% -98% and 85% - 106% with RSDs （n =5） of 5.1%, 5.6% and 7.7% at the spiked levels of 2 mg/kg, 0. 2 mg/kg and 0. 05 mg/kg, respectively. The minimum detection concentration of the method was 0. 014mg/kg.