坡柳皂苷对大菜粉蝶生长发育的抑制活性

Inhibitory activity of Dodonaea viscosa saponin on the growth and development of Pieris brassicae

采用浸渍叶碟饲喂法测定了坡柳皂苷对大菜粉蝶的生物活性。结果表明：用500、1625、2750、3875和5000μg／mL的坡柳皂苷处理24h后，对大菜粉蝶4龄幼虫生长发育的抑制率分别为14．47％、31．54％、49．70％、62．48％和79．94％，处理48h后的抑制率分别为2．72％、39．26％、54．82％、70．53％和86．39％；坡柳皂苷对大菜粉蝶4龄幼虫具有显著的拒食活性，供试5种浓度下处理24h后，拒食率分别为30．21％、40．62％、54．33％、72．47％和84．84％，48h后的拒食率为4．4％～86．67％；5000I,zg／mL处理14d后，幼虫化蛹率为32％．显著低于对照组（72％）；供试5种浓度坡柳皂苷处理23d后，蛹的羽化率分别为56％、44％、44％、36％和32％，均低于对照组（64％）。研究表明，坡柳皂苷对大菜粉蝶生长发育、取食及化蛹均有明显的抑制作用。

The biological activity of Dodonaea viscosa saponin to Pieris brassicae was determined by leaf dipping method. The results showed that the growth inhibition rates of 500, 1 625, 2 750, 3 875 and 5 000 ixg/mL D. viscosa saponin treatments on the 4th instar larvae of P. brassicae were 14.47%, 31.54%, 49.70%, 62.48% and 79.94% respectively, at 24 h, and were 2.72%, 39.26%, 54. 82%, 70. 53% and 86. 39% respectively, at 48 h. D. viscosa saponin had significantly antifeedant activity on the 4th instar larvae of P. brassicae, and the antifeedant rates of the 5 treatments of D. viscosa saponin mentioned above were 30.21%, 40. 62%, 54. 33%, 72. 47% and 84. 84% respectively, at 24 h, and were 4.4% - 86.67% at 48 h. The P. brassicae larvae pupate rate of 5 000 μg/mL treatment was 32% after 14 d, which was significantly lower than that of the control （72%）. And the P. brassicae emergence rate of the 5 treatments of D. viscosa saponin mentioned above were 56%, 44%, 44%, 36% and 32% respectively, after 23 d, which was lower than that of the control （64%）. The results showed that D. viscosa saponin had significant inhibitory activity on growth, development, feeding and pupation of P. brassicae.