关于抑制急性坏死性胰腺炎细菌移位的实验研究

Study on Inhibition of Bacterial Translocation in Acute Nnecrotizing Pancreatitis

目的:探讨利用不同方法抑制急性坏死性胰腺炎细菌移位的效果。方法:将60只SD大鼠分为对照组、ANP(急性坏死性胰腺炎)组、参附注射液组以及清胰利胆颗粒组,每组15只。利用牛磺胆酸钠制作SD大鼠的ANP模型,参附注射液组给予参附注射液进行治疗,清胰利胆颗粒组使用清胰利胆颗粒进行治疗,对照组与ANP组给予相同剂量的生理盐水。治疗结束后取大鼠的肠系膜淋巴结、腹水与下腔静脉血培养,对比四组大鼠各个部位的病原菌落计数。结果:对照组的各部位病原菌落计数要明显低于ANP组、参附注射液组与清胰利胆颗粒组(P<0.01);参附注射液组与清胰利胆颗粒组的各部位病原菌落计数要明显低于ANP组(P<0.01);参附注射液组与清胰利胆颗粒组的各部位病原菌落计数差异无统计学意义(P>0.05)。结论:参附注射液与清胰利胆颗粒可以有效地抑制急性坏死性胰腺炎的细菌移位。

Objective： To investigate the effect of different methods on inhibiting bacterial translocation in acute necrotizing pancreatitis （ANP）. Methods： 60 Sprague Dawley （SD） rats were divided into control group, ANP group, Shenfu injection group, and Qingyi gallbladder particle group, 15 for each group. Sodium taurocholate was employed to build the ANP model of SD rat. The rats in Shenfu group were treated with Shenfu injection, those in Qingyi gallbladder particle group were treated with Qingyi gallbladder particle, and those in control group and ANP group were given the same dose of saline After treatment, mesenteric lymph nodes, ascites, and inferior vena blood were acquired from rats and cultured, then, the pathogen colonies were counted and compared for rats in the four groups. Results：The bacteria colony counts from various sites in control group were significantly lower than those in ANP group, Shenfu group, and Qingyi gallbladder particle group （P〈0. 01）, The bacteria colony counts in Shenfu injection group and Qingyi gallbladder particle group were significantly lower than those in ANP group （P〈0. 01）. The pathogen colony counts in Shenfu group and Qingyi gallbladder particles group were not significantly different （P〉0. 05）. Conclusion： Shenfu injection and Qingyi gallbladder particles can effectively suppress bacterial transloeation in ANP.