抗肿瘤多肽蜂毒素的固相合成

Solid-phase synthesis of melittin of anti-tumor peptide

目的合成具有抗肿瘤活性的二十六肽蜂毒素。方法采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/DCC为缩合剂的合成工艺。结果通过本法能够顺利合成得到二十六肽蜂毒素,收率达32%。结论该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成。

Objective To synthesize the antitumor activity of twenty-six peptide melittin. Methods The melittin was synthe- sized by stepwise solid-phase peptide synthesis （ SPPS ）. During the synthesis of melittin, wang-resin was chosen as solid supporter and HOBt/ DCC were used as the coupling reagent. Results The melittin was synthesized with yield of 32%. Conclusion The syn- thetic method was feasible, simple and with high yield for long chain polypeptide synthesis.