肠道细菌对阿魏酸的代谢研究

Study on Metabolism of Ferulic Acid Produced by Intestinal Bacteria

研究人体肠道细菌对阿魏酸的代谢。采集健康志愿者新鲜粪便,称重稀释后厌氧培养,通过反复涂布分离得到69种人体肠道细菌。采用超高效液相四级杆飞行时间串联质谱技术(UPLC-Q-TOF/MS)与MetabolynxTM软件分析阿魏酸经人体粪便混合菌及分离的单菌作用后的代谢产物,共鉴定出了3种代谢产物,分别是氢化产物、去甲基化产物及去甲氧基化产物;此外,筛选出了对阿魏酸有特殊代谢活性的2种单菌,其中一种为已鉴定了的Bacillus sp.46;而另一种未知的细菌则用16S核糖体RNA(16S rRNA)技术进行了鉴定,结果表明,此细菌为Enterococcus sp.45。本研究提示肠道细菌对阿魏酸有代谢作用且由不同细菌完成。

The investigation on the metabolism of ferulic acid by incubating ferulic acid with different isolated bacteria from human feces in vitro was carried out. Fresh human fecal sample was obtained from a healthy volunteer, diluted serially in sterile water and sixty-nine different bacterial colonies were picked out ultimately. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q- TOF/MS） with automated data analysis software （ MetaboLynxTM ） was applied to fast analysis of [erulic acid metabolites. The results indicated that hydrogenation, demethylation and demethoxylation were the major metabolic pathways of ferulic acid by human intestinal bacteria in vitro. Two strain bacteria were found to metabolize ferulic acid and one of them had been identified as Bacillus sp. 46. The 16S rRNA gene of the other isolated bacterium sp. 45 was base pair sequences and its nucleotide sequence has registered in GenBank under accession no. KC41732. Finally, this strain was identified as Enterococcus sp. 45 by phylogenetic affiliation. This study suggested that intestinal bacteria had the metabolic effects of ferulic acid and the biotransformation was completed by different bacteria.