期刊文献+

TGF-β_1对绒毛膜癌JEG-3细胞基质金属蛋白酶-9基质金属蛋白酶抑制剂-1蛋白表达的影响 被引量:4

Effect of Transforming Growth Factor-β_1 on MMP-9 TIMP-1Protein Expression in JEG-3 Cells
下载PDF
导出
摘要 目的:观察转化生长因子-β1(transforming growth factor-β1,TGF-β1)对绒毛膜癌JEG-3细胞系基质金属蛋白酶-9(matrixmetalloproteinases,MMP-9)、基质金属蛋白酶抑制剂-1(TIMP-1)蛋白表达的影响。方法:体外培养人绒毛膜癌JEG-3细胞,分别给予不同浓度及不同作用时间的TGF-β1进行诱导,Western Blotting法检测MMP-9、TIMP-1蛋白的表达变化。结果:与对照组相比,100、200μg/L TGF-β1作用后,检测各组细胞MMP-9及TIMP-1蛋白的相对表达量均增加(P<0.01);与0h比较,12、24、48、72h各组细胞MMP-9及TIMP-1蛋白的相对表达量均增加(P<0.05),且各组MMP-9/TIMP-1大于1。结论:绒癌的浸润转移与MMP-9及TIMP-1的表达变化密切相关。 Objective: To investigate the effect of transforming growth factor-β1( TGF-β1) on matrixmetalloproteinases( MMP-9) and TIMP-1 protein expression in JEG-3 cells. Method: The human choriocarcinoma cell line cultured in vitro different doses of TGF-β1were given to JEG-3 cell at different time.The protein expression of MMP-9 and TIMP-1 induced by TGF-β1was measured by Western Blotting. Result: The protein expression of MMP-9 and TIMP-1 increased remarkedly,after inducing by TGF-β1with100μg / L and 200μg / L respectively,( P&lt;0.01). Conclusion: The invasive ability and metastasis of choriocarcinoma JEG-3 cells is closely related to the expression of MMP-9 and TIMP-1.
出处 《河北医学》 CAS 2014年第2期177-180,共4页 Hebei Medicine
基金 河北省自然科学基金项目(项目编号:H2012406010) 河北省教育厅课题资助项目(项目编号:2008303)
关键词 外源性TGF-Β1 绒癌JEG-3 MMP-9 TIMP-1 蛋白印迹法 Exogenous TGF-β1 Choriocarcinoma JEG-3 MMP-9 TIMP-1 Western blotting
  • 相关文献

参考文献7

  • 1孟海云,李福琴,赵琳琳,刘倩.MMP9、存活素在妊娠滋养细胞疾病中的表达及其临床意义[J].哈尔滨医科大学学报,2008,42(3):293-295. 被引量:2
  • 2Masatoshi Takahara,Takuji Naruse,Michiaki Takagi,Hiroshi Orui,Toshihiko Ogino.Matrix metalloproteinase-9 expression, tartrate-resistant acid phosphatase activity, and DNA fragmentation in vascular and cellular invasion into cartilage preceding primary endochondral ossification in long bones[J].Journal of Orthopaedic Research.2004(5)
  • 3E.Kerkel?,U.Saarialho‐Kere.Matrix metalloproteinases in tumor progression: focus on basal and squamous cell skin cancer[J].Experimental Dermatology.2003(2)
  • 4Aristidis Moustakas,Katerina Pardali,Annamaria Gaal,Carl-Henrik Heldin.Mechanisms of TGF-β signaling in regulation of cell growth and differentiation[J].Immunology Letters.2002(1)
  • 5J.A. Irving,P.K. Lala.Functional Role of Cell Surface Integrins on Human Trophoblast Cell Migration: Regulation by TGF-β, IGF-II, and IGFBP-1[J].Experimental Cell Research.1995(2)
  • 6Cairns RA,Khokha R,Hill RP.Molecular mechanisms of tumor invasion and metastasis: an integrated view[].Current Molecular Medicine.2003
  • 7Eliana Perissinotto,Giuliana Cavalloni,Francesco Leone,Valentina Fonsato,Stefania Mitola,Giovanni Grignani,Nadia Surrenti,Dario Sangiolo,Federico Bussolino,W,a Piacibello,Massimo Agliet.Involvement of Chemokine Receptor 4/Stromal Cell?Derived Factor 1 System during Osteosarcoma Tumor Progression[].Clinical Cancer Research.2005

二级参考文献9

  • 1傅晓华,石一复,崔金全.MMPs及TIMPs在滋养细胞疾病中表达的分析[J].实用妇产科杂志,2004,20(4):231-233. 被引量:6
  • 2Shih IM, Mazur MT, Kurman RJ. Gestational trophoblastic disease and related lesion. In: Kurman R J, ed. Blanstein' s Pathology of the Genital Tract [ M ]. 5th ed. New Nork : Springer Verlag, 2002 : 1 [93.
  • 3Denis LJ,Verweij J. Matrix metalloproteinase inhibitors: present achievements and future pospects[J]. Invest New Drugs, 1997, 15(3) : 175- 185.
  • 4Shimonovitz S, Hurwitz A, Dushnik M, et al. Developmental regulation of the expression of 72 and 92kd type IV collagenases in human trophoblasts: a possible mechanism for control of trophoblast invasion[J]. Am J Obstet Gynecol, 1994,171(3) :832-838.
  • 5Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma [ J ]. Nat Med, 1997, 3 ( 8 ) : 917-921.
  • 6Jeziorska M, Nagase H, Salamonsen LA, et al. Immunolocalization of the matrix metralloproteinases gelatinase B and strmelysin 1 in human endometrium throughout the menstrual cycle[ J ]. J Reprod Fertil, 1996, 107(1 ) :43-51.
  • 7Xu P, Wang YL, Zhu SJ, et al. Expression of matrix metrallopmteinases-2,-9, and -14, tissue inhibitors of metrallopmteinases-1, and matrix proteins in human placenta during the first trimester[J]. Biol Reprod, 2000,62(4) :988-944.
  • 8Altieri DC. The molecular basis and potential role of survivin in cancer diagnosis and therapy [ J ]. Trends Mol Med, 2001,7 ( 12 ) : 542-547.
  • 9Ka H, Hunt JS. Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas[J]. Am J Pathol,2003,163(2) :413- 422.

共引文献1

同被引文献147

引证文献4

二级引证文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部