大鼠脑出血后蛋白酶激活受体-1与细胞凋亡关系的实验研究

The experimental study about proteinase activated receptor-1 and apoptotic cell in rats after cerebral hemorrhage

目的研究大鼠脑出血(ICH)后血肿周围蛋白酶激活受体-1(PAR-1)、神经细胞凋亡等的表达。方法将60只SD大鼠分为假手术组和ICH组,采用立体定位仪定位注射自体不凝血制造大鼠ICH模型,分别于术后6h、1d、2d、3d、5d、7d处死,免疫组织化学法检测血肿周围PAR-1、TUNEL阳性细胞(凋亡细胞)数及小胶质细胞的表达。结果假手术组PAR-1少量表达,偶见凋亡细胞;ICH组自6h起PAR-1、凋亡细胞及小胶质细胞数量大量增加,3d达高峰,PAR-1在7d基本降至假手术组水平,但凋亡细胞、小胶质细胞仍处于较高水平,与假手术组比较差异有统计学意义(P<0.05)。结论 ICH后血肿周围存在大量PAR-1表达上调、神经细胞凋亡、小胶质细胞增加。

Objective To observe the expression of proteinase activated receptor-1（PAR-1） ,neuronal apoptotic cell in rats after cerebral hemorrhage .Methods 60 SD rats were randomly divided into 2 groups ,which were sham operation group （n=30） ,ICH group（n=30） .Autologous blood was injected into the caudate nucleus of rat that produced cerebral hemorrhage model by using stereotaxis instrument technique .Decapitation was conducted at 6 h ,1 d ,2 d ,3 d ,5 d and 7 d after the model was produced .Immu-nohistochemistry was used to detect PAR-1 positive cell ,TUNEL cell and Microglia in intracerebral hemorrhage group .Results There was only a few PAR-1 expression in sham operation group and few apoptotic cell （TUNEL positive cell） ,and there was no Microglia .Expression of PAR-1 positive cell ,TUNEL cell and Microglias in intracerebral hemorrhage group greatly increased from 6 h ,and reached a high level on the 3 d .PAR-1 positive cell descented to normal level at 7 d ,but TUNEL cell and microglias were expressed a higher level at 7 d ,there were statistical significance compared with sham operation （P〈0 .05） .Conclusion PAR-1 ,ap-optotic cell and microglia in the brian hematoma perienchyma markedly increased after ICH .