玉米自交系耐旱性的田间观察与分子标记检测

The Field Observation and Molecular Marker Detection of Maize Inbred Lines for Drought Tolerance

为了解玉米自交系耐旱性的田间鉴定与分子标记检测的一致性程度,从483份玉米自交系中筛选出田间耐旱性较好的17份,并在人为干旱胁迫下,观察鉴定了从国际玉米小麦改良中心引进的CML536、CML537、CML538和CML539的耐旱性。结果显示:CML537、CML538和CML539的耐旱性较好;玉米dhn1基因位点的CAPS标记dhnC397检测17份自鉴耐旱系和4份CIMMYT引进耐旱系的结果与田间耐旱性表现基本一致。用分子标记检测6份o2、o16和wx基因聚合系,其中4份不抗旱,表明有必要进一步聚合耐旱基因。结论,可利用分子标记技术初步检测玉米材料的耐旱性,此结果有利于促进分子标记辅助耐旱育种的开展。

To explore the degree of consistency of field observation and molecular marker detection of maize inbred lines for drought tolerance,2 1 maize inbred lines were observed for drought tolerance under field drought condition.The result showed that 17 lines,QCL2047,QCL2055,QCL2069,QCL2089, QCL2121,QCL2148,S611,HaiHe1,Pr（F2）（SV）,Pr（F2）（V）,CLQRCWQ26,CML491,QCL3053-7, CML535,CML537,CML538 and CML539,had better drought tolerance in the field.The drought tolerance of these lines was again detected by using CAPS marker of dhnC3 9 7 at dhn1 locus,and was basically consistent with the field performance, showing the molecular marker of dhnC3 9 7 could be preliminarily used for maize drought tolerance detection,and would help for promoting the development of molecular marker assisted breeding for drought tolerance.At the same time,6 lines pyramided o2、o1 6 and wx genes were tested with the marker,4 lines were not good for drought tolerance.Therefore,it is necessary to polymerize drought-tolerant gene further.