PRF植入兔拔牙创内的实验研究

Histological study on the impact of immediate implantation of Platelet Rich Fibrin on rabbit alveolarridge defect

目的探讨PRF对拔牙窝的促进骨再生作用及牙槽嵴保存的影响。方法①实验动物模型:将24只新西兰大白兔随机分为4组(A、B、C、D组),每组6只,拔除一侧下颌切牙。抽取大白兔自体外周血液,离心后制成PRF。A组拔牙窝内植入PRF/nano-HA混合物;B组拔牙窝内植入PRF;C组拔牙窝内植入nano-HA;D组拔牙窝作为空白对照。分别于术后4周、12周处死动物,取材。②观察:将标本脱钙脱水石蜡包埋行组织学切片,显微镜下观察骨愈合情况。结果术后4周,拔牙窝内新生骨组织数量质量A组>B组>C组>D组(空白组)。术后12周,A组成骨质量最好,B、C组骨改建情况类似,均优于D组。结论①PRF能够促进骨组织的修复。②PRF联合nano-HA具有保存拔牙窝牙槽嵴高度的功能,可作为牙槽骨保存的移植材料。

Objective To explore the role of PRF to promote bone regeneration of the sockets and the role to remain alveolar bone . Methods ①Experimental animal model：24 rabbits were randomly divided into 4 groups（group A, group B, group C, group D）, with one mandibular central incisors extracted .PRF was prepared by the autologous blood and implanted into the sockets .A, B,C group were respectively implanted with PRF/nano-HA, PRF,nano-HA composite graft, and D group was as control.Animals were killed respectively at 4, 12 weeks and samples were obtained for histological observation .②.Observation： Histological slides were made with decalcified , dehydrated and paraffin -embedded specimen ,and angiogenesis and osteosis were observed under microscope . Results In the 4th week after surgery , quantity and quality of new bone tissues group A ＆gt;group B ＆gt;group C ＆gt;group D.At 12 weeks the group A was better than other groups .There is no significant difference between the group B and group C .But both of them were better than qroup D .Conclusions ①PRF can accelerate healing of the bone tissue .②PRF/nano-HA play a role on preserving the alveolar ridge after teeth extraction and may be used as a favourable material on alveolarridge defect .