摘要
应用HPLC法研究水溶液中VC的稳定性。色谱条件:C18色谱柱;流动相为甲醇∶蒸馏水(3∶7,体积比);流速为1.0 mL/min;进样量:10μL;检测波长为260 nm。VC在20μg/mL^320μg/mL范围内线性关系良好;回归曲线为:y=2 008.3x-5 303.7;相关系数为R2=0.999 4。研究结果显示VC在水溶液中的衰减呈一级反应规律,半衰期为75 min。HPLC法适合研究水溶液中VC的稳定性。
To study the stability of vitamin C in aqueous solution with HPLC （high performance liquid chromatography） Method. Chromatography condition：C18column;using methanol∶distilled water（3∶7,volume ratio） as mobile phase;flow rate：1.0 mL/min;sample size：10μL;and the wavelength of detection was 260 nm. The linear relationship of VC displays good at 20μg/mL-320μg/mL;the regression curve：y=2008.3x-5303.7;the correlation coefficient is as R2=0.999 4. Results showed the attenuation of VC in aqueous solution was the property of first order reacting, and the half-life being 75 min. HPLC method was suitable to study the stability of VC in aqueous solution.
出处
《食品研究与开发》
CAS
北大核心
2014年第1期70-72,共3页
Food Research and Development
基金
遵义医学院中青年科研启动基金资助项目(F-500)
