中心组合设计法优化超声波提取芦荟甙工艺

Optimization of Ultrasonic-assisted Extraction of Aloin with Central Composite Design

以高效液相色谱法测定芦荟干粉中芦荟甙含量,运用中心组合设计对超声波辅助提取芦荟干粉中芦荟甙的工艺进行优化。结果表明:在乙醇浓度70%,液料比20∶1(mL/g),提取温度45℃下提取时间30 min,芦荟甙含量达到3.31 mg/g,与预测值3.33 mg/g相差不显著。

The best extracting conditions of Ultrasonic Assisted Extraction which obtained by Central Composite design were studied based on the amount of alion extractant measured by HPLC. The results showed that when the concentration of ethanol was 70 % ,liquid-solid ratio was 20：1 （mg/g） ,the extracting temperature was 45℃ and extracting time was 30 rain, the yield of aloin was 3.31 mg/g, which was very close to the predicted value of 3.33 mg/g.