siRNA转染浓度和时间对HeLa细胞ANXA5表达的抑制作用

INHIBITING EFFECTS OF SIRNA TRANSFECTION CONCENTRATION AND TIME ON ANXA5 EXPRESSION IN HELA CELLS

目的:优化靶向ANXA5的siRNA转染HeLa细胞的条件,为进一步研究ANXA5低表达对宫颈癌细胞生物功能的影响奠定基础。方法:将靶向ANXA5的siRNA转染HeLa细胞,设置不同的siRNA与脂质体的比值和转染时间,Western Blot检测ANXA5蛋白的表达。结果:siRNA与脂质体的比值为0.4:1时,转染入HeLa细胞72h后,HeLa细胞ANXA5蛋白的表达水平最低,即抑制作用最强。结论:siRNA与脂质体的比值和转染时间可影响HeLa细胞ANXA5的表达,存在最佳比值和转染时间。

Objective：To optimize the conditions of siRNA targeting ANXA5 transfected into HeLa cells, so to provide bases for further studying effects of ANXA5 low expression on biological functions of cervical cancer cells. Methods：siRNAtargetingANXA5 was transfected into HeLa cells, with different ratios ofANXA5 siRNAand liposomes, and different transfection time. Western Blot was used to detect the ANXA5 protein expression in HeLa cells. Results：The ANXA5 protein expression in HeLa cells was the lowest （the inhibitory effects was the strongest） when the ratio of siRNA and liposomes was 0.4：1 and transfection time was 72h. Conclusions：The ratio of siRNA and liposomes and transfection timecaninfluencetheexpressionofANXA5inHeLa cells, and has the best ratio and transfection time.