溶血对时间分辨免疫荧光分析法测定胰岛素的影响

Influence of hemolysis on concentrations of insulin measured by time-resolution fluoroimmunoassay analysis

目的探讨溶血对时间分辨免疫荧光分析法测定胰岛素的影响。方法收集30例未发生溶血的血清标本,利用低渗溶血法分别制备轻度溶血、中度溶血、重度溶血标本各10例;采用时间分辨免疫荧光分析法分别测定各组标本溶血前(0h)和溶血后0.5、2、18h的胰岛素浓度;通过配对t检验、重复测量数据的方差分析对溶血程度和时间对胰岛素浓度检测结果的影响进行分析。结果轻度溶血、中度溶血、重度溶血标本之间胰岛素浓度检测结果比较差异无统计学意义(P>0.05);随着溶血时间的延长,胰岛素浓度呈明显下降趋势,0.5、2、18h检测结果与0h检测结果相比,比较差异均有统计学意义(P<0.05)。结论溶血程度对时间分辨免疫荧光分析法测定胰岛素浓度的影响不明显,但溶血时间则对测定结果具有较为明显的影响;标本溶血和溶血时间均为影响胰岛素浓度检测结果的重要因素。

Objective To investigate the influence of hemolysis on concentrations of insulin measured by time-resolution immunofluorescence analysis （TRFIA）.Methods A total of 30 serum samples without hemolysis were se-lected,and mild hemolysis,moderate hemolysis,severe hemolysis samples,10 cases for each,were prepared by using hypotonic hemolysis method.TRFIA was performed to measured insulin concentrations before hemolysis （0 h）and 0.5,2,18 h after hemolysis.Variance analysis of repeated measurement data and paired t test were used to analyze the influence of hemolysis and time on insulin concentration.Results Insulin concentration in mild hemolysis,mod-erate hemolysis and severe hemolysis samples were without significant difference （P＆gt;0.05）.With the prolong of hemolysis time,the concentration of insulin was significantly decreased,compared between 0.5,2,18 and 0 h,the differences were significant （P＆lt;0.05）.Conclusion Effects of hemolysis on insulin concentration detected by TR-FIA might not be obvious,but the influence of hemolysis time could be significant.Hemolysis and hemolysis time might both be important factors,affecting the determination of insulin concentration.