抵挡汤对2型糖尿病大鼠胰岛素抵抗影响的实验研究

Effect of Didang decoction on insulin resistance in rats with type 2 diabetic

[目的]探讨抵挡汤对糖尿病大鼠大血管病变的作用及其机制。[方法]将自发2型糖尿病大鼠模型随机分为模型组、抵挡汤组、罗格列酮组,同系非糖尿病大鼠为空白对照组。抵挡汤组与罗格列酮组大鼠分别给以抵挡汤与罗格列酮灌胃,模型组与空白组均以等量蒸馏水灌胃。实验周期为12周,对大鼠进行葡萄糖曲线下面积,血脂、胰岛素抵抗指数、高胰岛素正糖钳夹实验与骨骼肌腺苷酸活化蛋白激酶(AMPK)活性定量检测。[结果]与模型组比较,抵挡汤组和罗格列酮组胰岛素抵抗指数不同程度下调(P<0.05)。与模型组比较,罗格列酮组甘油三酯及游离脂肪酸下调,抵挡汤组游离脂肪酸较模型组下调(P<0.05)。与模型组比较,罗格列酮组AMPK酶活性上升(P<0.05)。[结论]抵挡汤对糖尿病大鼠的血糖、血脂无明显影响,但可明显影响胰岛素抵抗指数与正糖钳夹实验葡萄糖输注率,提示其对胰岛素抵抗有较好的干预作用。

[Objective] To investigate the effect and its mechanism of Didang decoction on vascular lesions in diabetic rats. [Methods] Rats with type 2 diabetic were randomly divided into model group, Didang decoction group, rosiglitazone group, and LETO control group. The rats in Didang decoction group and the rosiglitazone group were treated orally with Didang decoction and rosiglitazone respectively, and the rats in model group and blank group with equal amount of distilled water. The experimental period was 12 weeks. The area under the curve of glucose, lipids, insulin resistance index, hyperinsulinemic euglycemic clamp test of skeletal muscle and the activity of AMPK quantitative detection of all the rats were detected. [Results] Compared with the model group, the insulin resistance index of the rats in Didang decoction group and rosiglitazone group was reduced （P〈0.05）. Compared with the model group, the triglyceride and free fatty acid of the rats were reduced in rosiglitazone group, and the free fatty acids of the rats were lowered in Didang decoction group （P〈0.05）. Compared with the model group, the AMPK activity of rats was increased in rosiglitazone group （P〈0.05）. [Conclusion] The Didang decoction has no effect on the blood glucose and blood lipid of rate with diabetic disease, but can significantly alter the insulin resistance index and euglycemic clamp glucose infusion rate and has effect on the insulin resistance of rats with type 2 diabetic.