仪器检测幼稚粒细胞与显微镜方法的比较

Comparison of instrument detecting immature granulocyte and microscopic counting

目的评价Sysmex XE-5000、XT-4000i血细胞分析仪测定幼稚粒细胞百分数与显微镜计数方法的一致性。方法随机抽取274例血液标本,分别在2 h内用XE-5000与XT-4000i测定幼稚粒细胞百分数,并采用SP1000i全自动推片机制备血涂片进行显微镜计数,对仪器检测结果和显微镜复检结果进行相关分析。结果 XE-5000与XT-4000i的测定结果和显微镜计数幼稚粒细胞百分数均显示有较好的相关性(R25000=0.968 9,R24000i=0.960 3)。结论 XE-5000与XT-4000i自动检测幼稚粒细胞的准确性均较高,只要按照复检规则对血细胞分析结果进行复检,幼稚粒细胞参数完全可直接应用于临床。

[ Objective ] To evaluate the uniformity of immature granulocyte detection by Sysmex XE-5000 hematology analyzer, XT-4000i hematology analyzer and microscopic counting. [ Methods] A total 274 blood samples were randomly collected and detec- ted respectively with XE-5000 and XT-4000i for the percentage of immature granulocyte within 2 hours. The blood smears were re- viewed by Sysmex SP1000i. Analysis of correlation was made between the two hematology analyzers ＇ results and microscopic count- ing. [ Results ] The results of Sysmex XE-5000 and XT- 4000i had good correlations with microscopic counting （ R25000 = 0. 968 9, R24000i = 0.960 3 ）. [ Conclusion] The results of immature granulocyte detected by Sysmex XE-5000 and XT-4000i hematology analyzers are very accurate. The parameter of immature granulocyte can be directly used in clinical work, by carrying out results re- view in accordance with the criteria.