芹菜素电化学探针与核酸作用机理的研究

Study on the Interaction Mechanism of Apigenin Electrochemical Probe with Nucleic Acid

通过电化学法研究了fsDNA影响下芹菜素（API）在金电极上的电极行为，优化确定了fsDNA的检测条件。结果表明，在4．0×10^-7～7．0×10^-6g／mL浓度范围内峰电流的增强与加入的fsDNA的浓度呈线性关系，线性方程为△Ip／（μA）=3．20CfsDNA（10^-5g／mL）＋1．06，检测限为5．8×10^-8g／mL，对模拟样品的测定结果令人满意；研究还表明芹菜素发生了3e传递行为，并有质子参与，且该行为受到扩散控制。通过循环伏安、紫外一可见光谱等方法对API与fsDNA间的作用机制进行了初步探讨，结果表明两者之间存在着静电作用，结合常数K=1．8×10^4L／mol。

In this paper, the electrode behavior of apigenin （API） on the gold electrode under the influence of fsDNA was investigated by electrochemical methods. The optimum conditions of fsDNA detection were studied. The results showed that the peak current was enhanced linearly with increasing of the fsDNA concentration in the range of 4.0 × 10 -7 - 7.0 ×10 -6g/mL. The linear equation was△Ip/（μA） = 3.20CfsDNA （ 10 -Sg/mL） ＋ 1.06. The detection limit was 5.8 × 10 -8g/mL. The nucleic acid content of simulated sample was successfully detected by this method. It was also found that there were 3e transfer behavior of API on gold electrode, and the protons were also involved in the electrode reaction process. The redox behavior on gold electrode was controlled by diffusion effect. The results obtained by cyclic voltammetry and UV-Vis spectroscopy indicated that there was electrostatic interaction between them. The binding constant was K = 1.8 × 104L/tool.