摘要
目的:探讨调节性T细胞在Polyinosinic-polycytidylic acid(poly I:C)/D-galactosamine(D-GalN)诱发的急性肝脏损伤中的作用及其机制。方法:建立poly I:C/D-GalN诱发的爆发性肝炎小鼠模型,观察比较野生型C57BL/6小鼠、Rag1-/-小鼠(C57BL/6背景)和过继转输调节性T细胞的Rag1-/-小鼠的血清转氨酶活性;肝脏HE染色评价小鼠肝组织病理形态学改变;实时荧光定量PCR检测肝脏TNF-α和IFN-γmRNA水平;ELISA检测血清IFN-γ和TNF-α蛋白水平。结果:poly I:C/D-GalN联合注射后,与野生型小鼠相比,Rag1-/-小鼠血清转氨酶水平明显增高,肝脏损伤更加严重,炎性因子mRNA水平和蛋白水平都显著增加。给Rag1-/-小鼠过继转输CD25+细胞后能明显降低poly I:C/D-GalN诱导的肝脏损伤。结论:调节性T细胞对poly I:C/D-GalN诱发的肝脏损伤有抑制作用,提示调节性T细胞可以负向调节天然免疫细胞。
Objective：To explore the role of regulatory T cells （Tregs） in liver injury induced by poly I： C/D-GaIN. Methods： In this study, C57BL/6 mice and Ragl-/- mice were injected with poly I： C/D-GaIN. Liver injury was evaluated by serum transaminase activity and histopathologic changes, mRNA levels of TNF-α and IFN-γ in the liver were measured by real time quantitative PCR, and their protein levels were determined by ELISA. Results： After injection of poly I：C/D-GalN, Ragl -/- mice showed higher ALT activity and more serious liver injury than C57BL/6 mice. And mRNA and protein levels of inflammatory cytokine were sig- nificantly increased in Ragl -/- mice. Transfer of CD25 ＋ cells significantly reduced liver injury in poly I ： C/D-GaiN-treated Ragl -/- mice. Conclusion： Tregs can suppress poly I： C/D-GaiN-induced liver injury, which indicates that Tregs may negatively regulate the response of natural immune cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第1期24-29,共6页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81001321
30972569)资助
