人工低温胁迫下西洋杜鹃生理生化指标的变化

Changes of Physiological and Biochemical Indexes of Rhododendron hybridum Under Artificial Low Temperature Stress

为了解西洋杜鹃的抗寒机制,以西洋杜鹃‘粉红’品种为实验材料,研究低温胁迫条件下‘粉红’叶片的相对电导率、丙二醛(MDA)含量、可溶性糖含量、脯氨酸含量、可溶性蛋白含量、SOD活性、CAT活性、POD活性等生理指标的变化情况。从图表可以看出:随着胁迫时间的延长,脯氨酸含量变化不明显,基本维持在正常水平;相对电导率、丙二醛含量较正常水平有缓慢上升趋势;可溶性糖含量、可溶性蛋白含量,SOD活性、CAT活性、POD活性总体呈持续上升的变化规律;胁迫温度越低,相对电导率、丙二醛(MDA)含量、可溶性糖含量、脯氨酸含量、可溶性蛋白含量、SOD活性、CAT活性、POD活性越高,温度低于3℃,即对‘粉红’产生较严重伤害,且温度越低,伤害程度越严重,经过0℃低温胁迫4天,可大大提高‘粉红’的抗寒性。

In order to understand the physiological mechanism of cold tolerance on Rhododendron hybridum, taking the variety of ＇Fenhong＇ as materials to study the relative electric conductivity, MDA content, soluble sugar content, proline content, soluble protein content, SOD activity, CAT activity, POD activity and other physiological indexes under low temperature stress condition. From the chart we can know that with the stress time prolonged, the proline content kept steady, relative electric conductivity and MDA content increased gradually, soluble sugar content, soluble protein content, SOD activity, CAT activity and POD activity kept rising. The lower the temperature was, the higher the relative electric conductivity, MDA content, soluble sugar content, proline content, soluble protein content, SOD activity, CAT activity and POD activity would be. When the temperature was below 3℃, ＇Fenhong＇ suffered serious damage, the lower the temperature was, the more serious degree of damage, after four days low temperature stress under 0℃, the cold acclimation of ＇ Fenhong＇ was greatly improved.