期刊文献+

外源乙烯调控大豆花粉育性的研究 被引量:3

Studies on pollen fertility regulated by exogenous ethylene in soybean(Glycine max L.Merrill)
下载PDF
导出
摘要 使用质量浓度为400mg/L的乙烯利水剂对大豆进行喷施处理,探讨乙烯对大豆花粉育性的调节作用。结果表明,外源乙烯处理后,大豆植株的成花量与花粉数量极显著低于对照,而花粉败育率极显著高于对照,外源乙烯处理对大豆花粉育性的抑制作用十分明显;乙烯利与对照处理基因的差异表达谱及实时定量PCR研究结果表明,细胞应答饥饿、花粉管生长、花粉外壁形成等多个大豆花粉发育相关基因的表达出现显著差异;相同时间段相比,乙烯处理的花芽组织中可溶性糖、可溶性淀粉、超氧化物歧化酶与过氧化物酶活性显著低于对照,而丙二醛含量显著高于对照。本研究为外源乙烯制剂调控大豆育性研究提供了理论依据。 Heterosis is a very important genetic phenomenon , and its application can significantly improve yields and enhance resistance in many crops . The use of this important tool , however , is limited in soybean production because: 1 ) The small size of soybean flowers makes it difficult and expensive to emasculate and hybridize;2) There are only a limited number of male sterile lines available for hybrid seed production . Therefore , illuminating the mechanisms of male sterile in soybeans will benefit heterosis research of soybean . Previous studies showed that ethylene biosynthesis in the soybean flower was closely related with male sterility . However , the mechanisms that ethylene induced male sterility of soybean remains unknown . Thus ,exogenous ethylene treatment was carried out to study its fertility regulation role in soybean . Scanning electron microscopy (SEM) and palynology methods were used to study the effects of ethylene treatment ,and real time quantitative PCR ( RT-qPCR) was used to evaluate gene expression . The results showed that flower numbers and pollen amount were significantly decreased , and abortion ratio of pollen was significantly promoted in the ethylene treatment compared with the control . The results of difference gene expression profile and RT-qPCR of the control and exogenous ethylene treatments indicated that the expression of eight genes which are related to cellular response to starvation , pollen growth and pollen wall assembly was changed significantly in the ethylene treatment compared with the control . Soluble sugar and starch content , activities of superoxide dismutase and peroxidase were all significantly lower while malondialdehyde content was significantly higher in flower tissue after ethephon treatment than the control . In conclusion , it is confirmed that exogenous ethylene treatment will cause male sterility in soybeans , and the expression of eight genes responsible for pollen development has been significantly changed after ethylene treatment . Carbon starvation and proactive enzyme injury are involved in the process of male sterility formation . The study above provides a theoretical basis for future research on pollen sterility regulated by exogenous ethylene in soybean .
出处 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2014年第1期25-32,共8页 Journal of Zhejiang University:Agriculture and Life Sciences
基金 国家自然科学基金项目(31000691) 国家留学基金委项目(201208220004) 吉林省科技发展计划项目(201201084)
关键词 大豆 花粉 乙烯 育性 soybean pollen ethylene fertility
  • 相关文献

参考文献7

二级参考文献91

共引文献143

同被引文献31

引证文献3

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部