根肿病(Plasmodiophora brassicae)菌侵染对大白菜幼苗叶片膜脂过氧化的影响

The Effect of Clubroot(Plasmodiophora brassicae) Infection on Cabbage Seedling Leaf Lipid Peroxidation

旨在研究根肿病菌侵染过程中膜脂过氧化相关指标及保护酶类活性的变化。以感病(91-12B)和抗病(CR91-12B)大白菜为试验材料,采用注射法对其进行接菌,取样测定。91-12B和CR91-12B抗氧化酶类活性均表现为先升高后降低,但抗病CR91-12B对病菌胁迫反应迅速,防御及时,活性氧系统处于动态平衡。感病91-12B对病菌胁迫反应慢,防御不及时,活性氧系统平衡被破坏。这说明接菌后,抗病材料能及时产生超氧阴离子作为信号激发细胞的抗氧化反应。同时发现,接菌后第8天为抗病CR91-12B对病菌胁迫产生反应的时间。本试验验证了根肿病抗性与膜脂过氧化间的关系,为一些生长调节剂和人工合成的活性氧清除剂在抗根肿病上的应用提供了依据;

The aim was to study the membrane lipid peroxidation indicators and protective enzymes activity of the Chinese cabbage leaf under clubroot bacteria stress conditions.Taking the susceptible 91-12B and resistant CR91-12B cabbage as test material,shots was adopted to carry out the inoculation,and then determined by sampling.91-12B and CR91-12B activities of antioxidant enzymes showed increase and then decreased.The results showed that resistant CR91-12B responded quickly and timely defense to pathogens,reactive oxygen species in dynamic equilibrium.Susceptible 91-12B responded slowly,the defense was not timely;the balance of reactive oxygen system was broken.It also found that,8 days after the inoculation of the bacteria was the reaction time of resistance CR91-12B.The experiment verified the relationship between clubroot resistance and membrane lipid oxidation which provided basis for the applications of growth regulators,synthetic active oxygen scavenger in resistance to clubroot and early screening of CR91-12B.