18个甘肃地方梨品种S基因型鉴定及新S-RNase基因克隆

Identification of 18 S-genotypes and Cloning Novel S-RNase in Pyrus from Gansu

为鉴定甘肃地方梨品种的自交不亲和基因型(S基因型),利用梨自交不亲和基因的特异引物对18个梨品种基因组DNA进行特异PCR扩增,对扩增片段进行回收、克隆和测序,确定了14个梨品种的S基因型。结果如下‘:临夏香把’S12S21、‘甘谷黑梨’S16S54、‘康乐白果’Sb Si、‘武都甜梨’S26Si、‘临夏黄麻’S3Se、‘康乐酥木梨’S1Sh、‘临夏萨拉’S16Se、‘九泉长把梨’S6S22、‘临姚麻甜梨’S22Sd、‘九泉麦梨’S6S17、‘漫路窝窝果’S12S21、‘武山糖梨’S8S19、‘康乐甘长把’S22Sd、‘河政甘长把’S22Sd。品种‘甘谷香水’和‘礼县新八盘’含有Se基因,‘临夏大吊蛋’和‘甘谷红霞’分别含有1个S26、S16基因。在‘甘谷黑梨’中发现1个新S等位基因,暂定名S54。该结果为研究梨属不同种间S-RNase基因的进化、田间授粉树的配置和新品种的选育等提供参考。

To identify S-genotypes of a few types of wild species originating from Gansu Province,S-RNase specific PCR were used.After cloning and sequencing of PCR products,the S-genotypes of 18 species native to Gansu were determined.The results were as follows：‘Linxiaxiangba＇ S12S21,‘Ganguheili＇ S16S54,‘Kanglebaiguo＇ Sb Si,‘Wudutianli＇ S26 Si,‘Linxiahuangma＇ S3Se,‘Kanglesumuli＇ S1 Sh,‘Linxiasala＇ S16Se,‘Jiuquanchangbali＇ S6S22,‘Linyaomatianli＇ S22Sd,‘Jiuquanmaili＇ S6S17,‘Manluwowoguo＇ S12S21,‘Wushantangli＇ S8S19,‘Kangleganchangba＇ S22 Sd,‘Hezhengganchangba＇ S22Sd.‘Ganguxiangshui＇and ‘Lixianxinbapan＇ had a Se-RNase gene.There was a S26 in‘Linxiadadiaodan＇ and S16 in‘Ganguhongxia＇.Subsequent sequence analysis showed that there was a novel S-RNase in‘Ganguheili＇,which was named S54.The results would be useful for research of evolution of genes between different pear species,selection of pollinizers and design of crossing in cultivar improvement of pear.