PVA降解菌株的筛选鉴定及其固定化工艺研究

Isolation,Identification and Immobilized of Efficient PVA-degrading Bacteria

为了得到一株降解PVA的芽孢杆菌,并对其进行固定化研究。利用PVA碘-硼酸平板法初筛,降解率测定法进行复筛,并通过正交试验探索其固定化工艺。经初筛得到8株可降解PVA的细菌,复筛出1株具有较高降解率的菌株3-9,48 h降解率为86.56%。该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis subsp.subtilis)。该菌株的最佳固定化条件为:4%海藻酸钠,3%CaC12,包埋0.1 g的湿菌体,钙化时间4 h。结果表明固定化后的PVA降解菌比游离菌的降解性能提高了14.02%。本研究得到了一株高效降解聚乙烯醇的枯草芽孢杆菌,并确定其最佳的固定化条件,为PVA污水处理提供了菌种资源和理论基础。

In order to get a strain of degrading PVA bacillus,and immobilization of the strain resources. Using PVA iodine-boric acid plate method for screening,measures degradation for rescreening,and explored the immobilization process by orthogonal experiment. Eight bacterial strains showed degrade activity to polyvinyl alcohol,and 3-9 was obtained during the rescreening. After 48 hours,PVA degradation rate up to 86.56%. This strain was identified as Bacillus subtilis subsp.. The optimum immobilization conditions for this strain are as follows： sodium alginate of 4%,CaC12 of 3%,cells of 0.1 g and calcification time was 4 hours. The results showed that degradation rate increased by 14.02% after immobilized.This research was supported by an efficient degradation of polyvinyl alcohol Bacillus subtilis subsp.,and determined the optimal immobilization conditions,provided resources and theoretical basis for PVA wastewater treatment.