摘要
从不同土壤样品中分离得到一株低温产纤维素酶高活性的细菌,并对其液态发酵条件进行优化。结果表明,此菌株液体发酵培养基最佳配方为:蛋白胨15.0g,羧甲基纤维素钠(CMC-Na)10.0g,秸秆粉10.0g,氯化钠5.0g;最佳培养条件为:培养基pH为6.0,发酵时间为4d,发酵温度为20℃,接种量为4%,摇床转速为180r/min;发酵条件优化后发酵液中纤维素酶活(CMCA)为38.96U/mL,是优化前的2.2倍。
A low temperature strain bacteria producing cellulose was isolated from variety of soil samples , and the liquid fermentation conditions were optimized. The results of experients showed that the primum culture medium were consisted with peptone 15.0g, carboxymethyl cellulose sodium （CMC-Na）10.0g, straw powder 10.0g; optimal culture conditions were fermentation medium pH of 6.0, fermentation time of 4d, fermentation temperature 20℃, 4% inoculated quantity, table speed of 180r/min. Under the optimum conditions, the cellulase activity （CMCA） of the fermented liquid was 38.96U/mL, and was 2.2 times as before.
出处
《食品与发酵科技》
CAS
2014年第1期38-41,共4页
Food and Fermentation Science & Technology
关键词
低温
纤维素酶
细菌
发酵条件
low temperature
cellulase
bacteria
fermentation conditions
