双歧杆菌与乳酸杆菌混合发酵条件的优化研究

Study on Optimum the Mixed Fermentation Conditions of Bifidobacterium and Lactobacillus

本文通过正交试验,首先优化双歧杆菌和乳酸杆菌混合发酵工艺,确定得出其最适发酵条件为:初始pH值为7.2,培养温度为35℃,最佳培养基配方为酵母膏0.9%,胰蛋白胨0.6%,大豆蛋白胨0.3%,葡萄糖2.5%,双歧因子(低聚果糖)0.3%,生长因子5%。在此基础上,在100L发酵罐规模,进行了乳酸菌和双歧杆菌通过上述条件混合发酵中实验试,实验结果显示,发酵液中双歧杆菌、乳酸杆菌发酵液的活菌数均达到每mL在10亿/mL个以上,可以满足产业化需要。

The optimum formula fermented by Bifidobacteria and Lactobacteria together was optimized by orthogonal experiment in this paper. The optimal fermentation condition were obtained, which involved 35℃ fermentation temperature, pH 7.2, the optimum composition for the fermentation medium was： yeast extract 0.9%, tryptone 0.6%, soy peptone 0.3%, glucose 2.5%, bifidus factor （fructo-oligose） 0.3%, growth factor 5%. Cultivated in 10L and 100L fermentor under the optimal fermentation condition the viable counts of Bifidobacterium and Lactobacillus reached up to 109CFU/mL, which can absolutely meet the needs of the industrialization.