正交优化微波辅助提取马兰叶总黄酮工艺的研究

Microwave Assisted Extraction of Total Flavonoids from Kalimeris indica(Linn.) Sch Leaves with Orthogonal Test

采用微波辅助提取马兰叶中的黄酮类化合物,并利用单因素试验和正交试验法对提取工艺参数进行优化。结果表明,各因素影响大小为:乙醇浓度>提取时间>料液比>提取功率;马兰叶总黄酮提取的最佳工艺为:乙醇浓度80%,料液比1∶25(g∶mL),提取功率300W,提取时间7min,总黄酮的提取率高达23.93%。利用微波辅助法提取马兰叶总黄酮类化合物,方法简便,提取率高。

Total flavonoids were extracted from Kalimeris indica （Linn.） Sch leaves by microwave assited extraction, and the processing factors were optimized by single factor and orthogonal design. The results showed the order of factors to affect the extraction was ethanol concentration〉extraction time〉ratio of sample to solvent〉microwave power. The optimized conditions for microwave-assisted extraction of flavonoids were concluded as follows： ethanol concentration 80%, the ratio of solvent to sample 1：25, microwave power 300W and extraction time 3rain, the highest extraction yield was 23.93%. The microwave assisted extraction technology of total flavonoids from Ka!imeris indica （Linn.） Sch leaves is simplicity, conveniency, and efficiency.