电针联合天麻多糖对脑缺血大鼠齿状回神经干细胞巢蛋白和干细胞因子表达的影响

Effects of Electroacupuncture Intervention Combined with Polysaccharide of Gastrodia elata Blume on Expression of Nestin and Cytokines of Neural Stem Cells in the Dentate Gyrus of Cerebral Ischemia Rats

目的:观察并比较电针联合天麻多糖与单独电针或天麻多糖治疗脑缺血大鼠对神经干细胞增殖的影响,探讨针药结合治疗脑缺血的机制。方法:将SD大鼠随机分为正常对照组、模型组、电针组、天麻多糖组和针药结合组,每组8只。以右侧大脑中动脉栓塞法制备脑缺血模型。造模后2周,天麻多糖组和针药结合组大鼠给予天麻多糖100mg/kg灌胃,每天1次,连续2周;电针组和针药结合组大鼠给予"百会"、左侧"足三里"穴电针刺激,每次持续30min,每天1次,连续2周。采用免疫组织化学染色法结合图像分析检测大鼠齿状回(DG)的内源性神经干细胞巢蛋白(Nestin)和干细胞因子(SCF)表达。结果:与正常对照组比较,模型组缺血侧DG的Nestin、SCF阳性表达增加(P<0.05);与模型组比较,电针组、天麻多糖组和针药结合组缺血侧DG的Nestin、SCF阳性表达明显增加(P<0.05);针药结合组阳性表达显著多于电针组或天麻多糖组(P<0.05)。结论:电针与天麻多糖结合可显著增加脑缺血大鼠缺血侧DG的Nestin、SCF表达,促进内源性神经干细胞增殖,且作用优于单用电针或天麻多糖。

Objective To observe the effect of electroacupuncture （EA） of “Baihui” （GV 20） and “Zusanli” （ST 36） and EA plus polysaccharide of Gastrodia elata Blume （PGB） on neural stem cell （NSC） proliferation in cerebral ischemia （el） rats, so as to explore its mechanisms underlying improvement of CI. Methods A total of 40 SD rats were randomly divided into normal control, CI model, EA , medication （PGB） and EA＋ medication groups （n=8 in each group）. The CI model was estab- lished by occlusion of the middle cerebral artery （MCAO）. Two weeks after modeling, rats of the medication and EA＋ medication groups were administrated with PGB （100 mg/kg） once daily for 2 successive weeks. EA （2 Hz, 3 V） was applied to “Baihui” （GV 20） and left “Zusanli” （ST 36） for 30 min, once daily for 2 weeks. The expression of Nestin and stem cell factor （SCF） pro- teins in the dentate gyrus （DG） of hippocampus was detected by immunohistochemistry. Results Compared with the normal control group, the expression of Nestin and SCF proteins in the suprapyramidal blade （SPB） and infrapyramidal blade （IPB） of DG were significantly increased in the model group （ P〈0.05）. After EA intervention, the expression levels of Nestin and SCF of SPB and IPB of DG were considerably further up-regulated in both EA group and EA＋ medication group （ P〈0.05）, and the effect of EA＋ medication group was significantly superior to that of simple EA group and medication group （P〈0.05）. Conclusion EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the hippocampal DG in CI rats, and its effects are apparently superior to those of simple EA or simple medication. It suggests that EA-induced proliferation of SCF may be one of the mechanisms underlying relieving CI.