糜酶途径对犬血管平滑肌细胞产生血管紧张素Ⅱ的影响

Effect of chymase on generation of Ang Ⅱ of cultured dog vascular smooth muscle cells

目的:探讨糜酶途径对犬血管平滑肌细胞(VSMC)产生血管紧张素Ⅱ(AngⅡ)的影响。方法:通过组织块贴壁法培养犬动脉VSMC,分为5组:对照组、AngⅠ组、卡托普利组、糜酶抑素组、联合用药组。对照组中仅为空白培养液,其他各组均加入AngⅠ,卡托普利组、糜酶抑素组和卡托普利+糜酶抑素组再分别加入相应的干预药物。应用放射免疫法检测培养液中AngⅡ含量等。结果:本实验成功培养出犬VSMC,含AngⅠ各组的AngⅡ浓度均高于对照组(P<0.05);卡托普利组的AngⅡ浓度减少,但与AngⅠ组比较差异无统计学意义(P>0.05);糜酶抑素组明显低于AngⅠ组和卡托普利组(P<0.05);卡托普利+糜酶抑素组作用更强一些,但与糜酶抑素组比较差异无统计学意义(P>0.05)。结论:糜酶在犬VSMC的AngⅡ生成过程中起着重要作用,其抑制剂可抑制AngⅡ生成。

Objective：To investigate the effect of chymase on generation of angiotensin Ⅱ （Ang Ⅱ ） of cultured dog vascular smooth muscle cells. Methods： The dog artery smooth muscle cells were cultured and divided into five groups： Control group; Angiotensin Ⅰ （Ang Ⅰ ） group;Captopril group; Chymostatin group; Captopril plus chymostatin group. Ang It concentration were measured in the culture medium. Results： The concentrations of Ang tl in every groups were higher than control group （P〈 0.05）; The concentration of Ang II in Captopril group decreased, but had no statistical significance （P 〉 0.05） compared with Ang Ⅰ group; Chymostatin group was lower than Ang Ⅰ group and captopril group（P〈 0.05）; Captopril ＋ Chymostatin group effect is stronger but has no statistical significance（P〉 0.05） compared with Chymostatin group. Conclusion： Chymase may play an important role in generation of Ang Ⅱ of cultured dog vascular smooth muscle cells. Chymostatin may inhibit generation of Ang Ⅱ.