卡托普利抑制胃癌细胞生长的体外研究

Inhibition of Gastric Cancer Cell Growth Induced by Captopril in vitro

背景:胃癌是我国常见的恶性肿瘤之一,血管紧张素转换酶抑制剂卡托普利对多种肿瘤具有抑制作用,但在胃癌中尚无研究。目的:探讨卡托普利对胃癌细胞增殖、凋亡和侵袭力的影响。方法:以不同浓度卡托普利作用于人胃癌细胞株AGS。采用CCK-8法检测细胞增殖率;采用DAPI染色法检测细胞凋亡表现;采用Annexin V-FITC/PI双染法、TUNEL法检测细胞凋亡率;采用Transwell法检测细胞侵袭力。结果:卡托普利作用后,AGS细胞增殖抑制,凋亡增加,细胞侵袭力降低,均呈浓度和(或)时间依赖性(P<0.05)。结论:卡托普利可抑制胃癌细胞株AGS增殖,诱导细胞凋亡,降低细胞侵袭力,效应呈浓度和(或)时间依赖性。

Background：Gastric cancer is one of the commonly seen malignant tumors of digestive system.Captopril,angiotensin-converting enzyme inhibitor,can inhibit the growth of many tumors,however,no study concerning gastric cancer has yet been reported.Aims：To investigate the effect of captopril on proliferation,apoptosis and invasiveness of gastric cancer cells.Methods：Human gastric cancer cell line AGS was treated with different concentrations of captopril.Cell proliferation rate was measured by CCK-8 assay.Manifestation of cell apoptosis was illustrated by DAPI staining.Cell apoptosis rate was measured by Annexin V-FITC/PI double staining and in situ end-labeling （TUNEL） method.Invasiveness of cell was determined by Transwell invasion assay.Results：Proliferation and invasiveness of AGS cells were inhibited and apoptosis was induced by captopril in a concentration and/or time-dependent manner （P ＜ 0.05）.Conclusions：Captopril can inhibit the proliferation and invasiveness and induce cell apoptosis of gastric cancer cell line AGS in a concentration-and/or time-dependent manner.